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Literature summary for 2.4.1.10 extracted from

  • Kirtel, O.; Menendez, C.; Versluys, M.; Van den Ende, W.; Hernandez, L.; Toksoy Oener, E.
    Levansucrase from Halomonas smyrnensis AAD6T first halophilic GH-J clan enzyme recombinantly expressed, purified, and characterized (2018), Appl. Microbiol. Biotechnol., 102, 9207-9220 .
    View publication on PubMed
Search for more literature for 2.4.1.10

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM EDTA enhances the enzyme activity by 31% Halomonas smyrnensis
NaCl the enzyme requires at least 1.5 M NaCl to be active and shows maximum activity in the presence of 3.5 M NaCl Halomonas smyrnensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Halomonas smyrnensis

General Stability

General Stability Organism
the enzyme is still very active at salt concentrations close to saturation (4.5 M NaCl) Halomonas smyrnensis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ complete inhibition at 5 mM Halomonas smyrnensis
Ca2+ 1% residual activity at 1 mM Halomonas smyrnensis
Co2+ complete inhibition at 1 mM Halomonas smyrnensis
Cu2+ complete inhibition at 1 mM Halomonas smyrnensis
Fe2+ complete inhibition at 1 mM Halomonas smyrnensis
KI
-
 Halomonas smyrnensis
Mg2+ 61% residual activity at 5 mM Halomonas smyrnensis
Mn2+ 6% residual activity at 5 mM Halomonas smyrnensis
Na2S2O5
-
 Halomonas smyrnensis
NaBr
-
 Halomonas smyrnensis
NaNO3
-
 Halomonas smyrnensis
Ni2+ complete inhibition at 1 mM Halomonas smyrnensis
SDS 2% residual activity at 5 mM Halomonas smyrnensis
Triton X-100 71% residual activity at 5 mM Halomonas smyrnensis

Organism

Organism UniProt Comment Textmining
Halomonas smyrnensis M1QZ44
-
-
Halomonas smyrnensis AAD6 M1QZ44
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Halomonas smyrnensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory Halomonas smyrnensis ?
-
-
additional information the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory Halomonas smyrnensis AAD6 ?
-
-
sucrose the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl) Halomonas smyrnensis levan + fructooligosaccharides
-
 ?
sucrose the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl) Halomonas smyrnensis AAD6 levan + fructooligosaccharides
-
 ?
sucrose + cellobiose
-
 Halomonas smyrnensis fructosylated cellobiose + ?
-
 ?
sucrose + cellobiose
-
 Halomonas smyrnensis AAD6 fructosylated cellobiose + ?
-
 ?
sucrose + galactose
-
 Halomonas smyrnensis Fru(beta-2,6)Gal + ?
-
 ?
sucrose + galactose
-
 Halomonas smyrnensis AAD6 Fru(beta-2,6)Gal + ?
-
 ?
sucrose + glucose
-
 Halomonas smyrnensis 1-kestotriose + 6-kestotriose + 1-kestotetraose + ?
-
 ?
sucrose + glucose
-
 Halomonas smyrnensis AAD6 1-kestotriose + 6-kestotriose + 1-kestotetraose + ?
-
 ?
sucrose + L-arabinose
-
 Halomonas smyrnensis 6-kestotriose + ?
-
 ?
sucrose + L-arabinose
-
 Halomonas smyrnensis 6-kestotriose + fructosylated L-arabinose
-
 ?
sucrose + lactose
-
 Halomonas smyrnensis fructosylated lactose + ?
-
 ?
sucrose + raffinose
-
 Halomonas smyrnensis Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Halomonas smyrnensis
? x * 47300, calculated from amino acid sequence Halomonas smyrnensis

Synonyms

Synonyms Comment Organism
Lsc
-
 Halomonas smyrnensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Halomonas smyrnensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
-
 Halomonas smyrnensis

pI Value

Organism Comment pI Value Maximum pI Value
Halomonas smyrnensis calculated from amino acid sequence
-
 4.6