Literature summary for 2.4.1.10 extracted from

Li, W.; Yu, S.; Zhang, T.; Jiang, B.; Stressler, T.; Fischer, L.; Mu, W.
Efficient biosynthesis of lactosucrose from sucrose and lactose by the purified recombinant levansucrase from Leuconostoc mesenteroides B-512 FMC (2015), J. Agric. Food Chem., 63, 9755-9763.

Search for more literature for 2.4.1.10

Application

Application	Comment	Organism
food industry	lactosucrose, a rare trisaccharide formed from sucrose and lactose by enzymatic transglycosylation, is a type of indigestible carbohydrate with a good prebiotic effect. Lactosucrose biosynthesis is efficiently carried out by a purified levansucrase from Leuconostoc mesenteroides B-512	Leuconostoc mesenteroides

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant His6-tagged enzyme expression in Escherichia coli strain BL21(DE3)	Leuconostoc mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	Q5IS34	-	-
Leuconostoc mesenteroides B?512	Q5IS34	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
791	-	purified recombinant His-tagged enzyme, pH 6.5, 50°C, transfructosylation with lactose as acceptor substrate	Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + lactose	optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme	Leuconostoc mesenteroides	D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside	i.e. lactosefructose or lactosucrose	?
sucrose + lactose	optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme	Leuconostoc mesenteroides B?512	D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside	i.e. lactosefructose or lactosucrose	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Leuconostoc mesenteroides

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Release 2023.1 (January 2023)