Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | levan synthesis from sucrose, structure analysis by NMR spectrometry, overview. Levan size analysis by gel filtration. As sucrose concentration increases, the average molecular weight of the low dextran distribution decreases, at 4°C compared to 37°C, the low molecular weight distribution region increases in size at any given substrate concentration, and a maximum of 20.64 kDa is obtained at 200 g/l | ? | - |
? | |
additional information | Bacillus subtilis 168 | levan synthesis from sucrose, structure analysis by NMR spectrometry, overview. Levan size analysis by gel filtration. As sucrose concentration increases, the average molecular weight of the low dextran distribution decreases, at 4°C compared to 37°C, the low molecular weight distribution region increases in size at any given substrate concentration, and a maximum of 20.64 kDa is obtained at 200 g/l | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P05655 | - |
- |
Bacillus subtilis 168 | P05655 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by cation exchange chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | levan synthesis from sucrose, structure analysis by NMR spectrometry, overview. Levan size analysis by gel filtration. As sucrose concentration increases, the average molecular weight of the low dextran distribution decreases, at 4°C compared to 37°C, the low molecular weight distribution region increases in size at any given substrate concentration, and a maximum of 20.64 kDa is obtained at 200 g/l | Bacillus subtilis | ? | - |
? | |
additional information | measurement of sucrose hydrolysis, releasing D-glucose and D-fructose. As the reaction proceeds, SacB becomes less hydrolytic while transferase becomes preponderant to reach up to 80% at the end of the reaction | Bacillus subtilis | ? | - |
? | |
additional information | levan synthesis from sucrose, structure analysis by NMR spectrometry, overview. Levan size analysis by gel filtration. As sucrose concentration increases, the average molecular weight of the low dextran distribution decreases, at 4°C compared to 37°C, the low molecular weight distribution region increases in size at any given substrate concentration, and a maximum of 20.64 kDa is obtained at 200 g/l | Bacillus subtilis 168 | ? | - |
? | |
additional information | measurement of sucrose hydrolysis, releasing D-glucose and D-fructose. As the reaction proceeds, SacB becomes less hydrolytic while transferase becomes preponderant to reach up to 80% at the end of the reaction | Bacillus subtilis 168 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SacB | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Bacillus subtilis |