Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits transfructosylation and hydrolytic activities | Bacillus velezensis | |
Fe2+ | inhibits transfructosylation and hydrolytic activities | Bacillus velezensis | |
Mn2+ | inhibits only the hydrolytic activity | Bacillus velezensis | |
N-bromosuccinimide | - |
Bacillus velezensis | |
Ni2+ | inhibits transfructosylation and hydrolytic activities | Bacillus velezensis | |
phenylmethanesulfonyl fluoride | - |
Bacillus velezensis | |
Zn2+ | inhibits transfructosylation and hydrolytic activities | Bacillus velezensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus velezensis | |
117.2 | - |
sucrose | pH 6.5, 40°C | Bacillus velezensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Bacillus velezensis | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates both transfructosylation and hydrolytic activities | Bacillus velezensis | |
Mg2+ | activates both transfructosylation and hydrolytic activities | Bacillus velezensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Bacillus velezensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n | Bacillus velezensis | - |
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1 | - |
? | |
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n | Bacillus velezensis SK 21.002 | - |
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus velezensis | - |
- |
- |
Bacillus velezensis SK 21.002 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native intracellular enzyme 11.8fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Bacillus velezensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
135.4 | - |
purified enzyme, pH 6.5, 40°C | Bacillus velezensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n | - |
Bacillus velezensis | D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1 | - |
? | |
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n | - |
Bacillus velezensis SK 21.002 | D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, intracellular enzyme, SDS-PAGE | Bacillus velezensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
transfructosylation activity | Bacillus velezensis |
45 | - |
hydrolytic activity | Bacillus velezensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
transfructosylation and hydrolytic activity | Bacillus velezensis |