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Literature summary for 2.4.1.10 extracted from

  • Nam, S.; Moon, Y.; Kang, J.; Kim, Y.; Robyt, J.; Kim, D.
    Synthesis, structural analysis and application of novel acarbose-fructoside using levansucrase (2009), Enzyme Microb. Technol., 45, 247-252.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene mlft, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
gene mlft
-
Leuconostoc mesenteroides B-512 FMC
-
gene mlft
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + acarbose
-
Leuconostoc mesenteroides D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose product analysis and structure determination by NMR spectroscopy ?
sucrose + acarbose
-
Leuconostoc mesenteroides B-512 FMC D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose product analysis and structure determination by NMR spectroscopy ?

Synonyms

Synonyms Comment Organism
M1FT protein
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Leuconostoc mesenteroides