Cloned (Comment) | Organism |
---|---|
gene mlft, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
gene mlft | - |
Leuconostoc mesenteroides B-512 FMC | - |
gene mlft | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + acarbose | - |
Leuconostoc mesenteroides | D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose | product analysis and structure determination by NMR spectroscopy | ? | |
sucrose + acarbose | - |
Leuconostoc mesenteroides B-512 FMC | D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose | product analysis and structure determination by NMR spectroscopy | ? |
Synonyms | Comment | Organism |
---|---|---|
M1FT protein | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Leuconostoc mesenteroides |