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Literature summary for 2.4.1.10 extracted from
- Heterologous expression and optimized one-step separation of levansucrase via elastin-like polypeptides tagging system (2007), J. Microbiol. Biotechnol., 17, 1751-1757.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lscA, expression in Escherichia coli DH5alpha | Pseudomonas chlororaphis subsp. aurantiaca |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
The molecular masses of levansucrase-elastin-like polypeptides [V-20] and levansucrase-elastin-like polypeptides [V-40] are determined as 56 kDa and 65 kDa, respectively. | Pseudomonas chlororaphis subsp. aurantiaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas chlororaphis subsp. aurantiaca | - |
gene lscA, expression in Escherichia coli DH5alpha | - |
| Pseudomonas chlororaphis subsp. aurantiaca S-4380 | - |
gene lscA, expression in Escherichia coli DH5alpha | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by applying elastin-like polypeptides tags for simple one-step purification based on temperature-triggered precipitation | Pseudomonas chlororaphis subsp. aurantiaca |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 171 | - |
levansucrase-ELP[V-40], measured spectrophotometrically | Pseudomonas chlororaphis subsp. aurantiaca |
| 173 | - |
levansucrase-ELP[V-20], measured spectrophotometrically | Pseudomonas chlororaphis subsp. aurantiaca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| levansucrase | - |
Pseudomonas chlororaphis subsp. aurantiaca |
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Release 2023.1 (January 2023)
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