Literature summary for 2.4.1.10 extracted from

Meng, G.; Fuetterer, K.
Donor substrate recognition in the raffinose-bound E342A mutant of fructosyltransferase Bacillus subtilis levansucrase (2008), BMC Struct. Biol., 8, 16.

Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli DH5alpha	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of the inactive single site mutants D86A, D247A and E342A of Bacillus subtilis levansucrase and from the sucrose- and raffinose-bound complexes of the E342A mutant, minimal structural consequences of the D247A, D86A mutations	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
D247A	single site mutant, substitutions have little effect on the active site geometry, catalytically inactive	Bacillus subtilis
D86A	single site mutant, substitutions have little effect on the active site geometry, catalytically inactive	Bacillus subtilis
E342A	single site mutant, catalytically inactive, the E342A mutant reveals conformational flexibility of functionally relevant side chains in the vicinity of the general acid Glu342, including Arg360, a residue required for levan polymerisation	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Bacillus subtilis	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + (2,6-beta-D-fructosyl)n	Bacillus subtilis	-	D-glucose + (2,6-beta-D-fructosyl)n+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P05655	expression in Escherichia coli DH5alpha	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-raffinose	-	Bacillus subtilis	D-fructose + melibiose	-	?
sucrose + (2,6-beta-D-fructosyl)n	-	Bacillus subtilis	D-glucose + (2,6-beta-D-fructosyl)n+1	-	?

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Release 2023.1 (January 2023)