Cloned (Comment) | Organism |
---|---|
Escherichia coli strain DH5alpha is used for cloning procedures using a pET3a plasmid and Escherichia coli strain JM109 is used for heterologous protein expression | Limosilactobacillus panis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | incubation with EDTA blocks enzyme activity completely | Limosilactobacillus panis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.5 | - |
sucrose | - |
Limosilactobacillus panis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Mg2+, Zn2+, Mn2+, Fe2+ have no effect | Limosilactobacillus panis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | Limosilactobacillus panis | - |
D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus panis | - |
The amino acid sequence of Lactobacillus panis levansucrase shows highest similarity to the characterized levansucrases of Lactobacillus sanfranciscensis (67%) and Lactobacillus reuteri (70%); TMW1.648 (isogenic to strain DSM 6035), expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
HisTrap purification | Limosilactobacillus panis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | - |
Limosilactobacillus panis | D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + lactose | - |
Limosilactobacillus panis | D-glucose + beta-D-fructofuranosyl-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside | - |
? | |
sucrose + maltose | - |
Limosilactobacillus panis | ? | - |
? | |
sucrose + raffinose | - |
Limosilactobacillus panis | melibiose + ? | - |
? | |
sucrose + sucrose | product concentrations of fructose, kestose or fructan depend on the concentration of sucrose, pH and temperatur | Limosilactobacillus panis | ? | - |
? | |
sucrose + sucrose | high concentration of sucrose | Limosilactobacillus panis | kestose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FTF | - |
Limosilactobacillus panis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | sodium acetate pH 4.1 containing 5 mM CaCl2 | Limosilactobacillus panis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
50% activity is reached at 40°C | Limosilactobacillus panis |
50 | - |
above 50°C a sharp loss of activity is observed | Limosilactobacillus panis |
60 | - |
at 60°C practically no activity is detectable | Limosilactobacillus panis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.6 | 45°C, optimal enzymatic activity is observed in this range, the influence of the buffering substance is not signifcant | Limosilactobacillus panis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | 50% of the maximal activity are detected at pH 3 and pH 5.5, residual activity is measured at pH 7.5 | Limosilactobacillus panis |