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Literature summary for 2.4.1.10 extracted from

  • Waldherr, F.W.; Meissner, D.; Vogel, R.F.
    Genetic and functional characterization of Lactobacillus panis levansucrase (2008), Arch. Microbiol., 190, 497-505.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli strain DH5alpha is used for cloning procedures using a pET3a plasmid and Escherichia coli strain JM109 is used for heterologous protein expression Limosilactobacillus panis

Inhibitors

Inhibitors Comment Organism Structure
EDTA incubation with EDTA blocks enzyme activity completely Limosilactobacillus panis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
22.5
-
sucrose
-
Limosilactobacillus panis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Mg2+, Zn2+, Mn2+, Fe2+ have no effect Limosilactobacillus panis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n Limosilactobacillus panis
-
D-glucose + (2,6-beta-D-fructosyl)n+1
-
?

Organism

Organism UniProt Comment Textmining
Limosilactobacillus panis
-
The amino acid sequence of Lactobacillus panis levansucrase shows highest similarity to the characterized levansucrases of Lactobacillus sanfranciscensis (67%) and Lactobacillus reuteri (70%); TMW1.648 (isogenic to strain DSM 6035), expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap purification Limosilactobacillus panis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Limosilactobacillus panis D-glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + lactose
-
Limosilactobacillus panis D-glucose + beta-D-fructofuranosyl-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside
-
?
sucrose + maltose
-
Limosilactobacillus panis ?
-
?
sucrose + raffinose
-
Limosilactobacillus panis melibiose + ?
-
?
sucrose + sucrose product concentrations of fructose, kestose or fructan depend on the concentration of sucrose, pH and temperatur Limosilactobacillus panis ?
-
?
sucrose + sucrose high concentration of sucrose Limosilactobacillus panis kestose + D-glucose
-
?

Synonyms

Synonyms Comment Organism
FTF
-
Limosilactobacillus panis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 sodium acetate pH 4.1 containing 5 mM CaCl2 Limosilactobacillus panis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
50% activity is reached at 40°C Limosilactobacillus panis
50
-
above 50°C a sharp loss of activity is observed Limosilactobacillus panis
60
-
at 60°C practically no activity is detectable Limosilactobacillus panis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.6 45°C, optimal enzymatic activity is observed in this range, the influence of the buffering substance is not signifcant Limosilactobacillus panis

pH Range

pH Minimum pH Maximum Comment Organism
3 7.5 50% of the maximal activity are detected at pH 3 and pH 5.5, residual activity is measured at pH 7.5 Limosilactobacillus panis