H296C |
site-directed mutagenesis, no detectable activities |
Zymomonas mobilis |
H296E |
site-directed mutagenesis, no detectable activities |
Zymomonas mobilis |
H296K |
site-directed mutagenesis, minimal hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 4.6% of the wild-type |
Zymomonas mobilis |
H296L |
site-directed mutagenesis, minimal hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 1.0% of the wild-type, mediates the accumulation of low amounts of trisaccharide, but did not produce pentasaccharide |
Zymomonas mobilis |
H296Q |
site-directed mutagenesis, minimal hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 2.5% of the wild-type |
Zymomonas mobilis |
H296R |
site-directed mutagenesis, retains partial hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 23.1% of the wild-type |
Zymomonas mobilis |
H296S |
site-directed mutagenesis, no detectable activities |
Zymomonas mobilis |
H296W |
site-directed mutagenesis, retains partial hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 19.8% of the wild-type |
Zymomonas mobilis |
H296Y |
site-directed mutagenesis, no detectable activities |
Zymomonas mobilis |
additional information |
The His296 mutation influences the substrate recognizing and binding, and then has an effect on sucrose hydrolysis and fructosyl-enzyme intermediate formation. Its mutations weakens the transfructosylation reaction and changes product specificities |
Zymomonas mobilis |