Cloned (Comment) | Organism |
---|---|
expression in Actinomyces naeslundii strain Lev1 | Actinomyces naeslundii |
overexpression as His-tagged protein in Escherichia coli strain His-Is M15 and DH10B, partial gene ftf without the predicted signal sequence | Actinomyces naeslundii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | allelic mutagenesis of wild-type strain, inactivation of ftf gene, no remaining levansucrase activity in the mutated strain | Actinomyces naeslundii |
T14V | levanase-deficient mutant | Actinomyces naeslundii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | - |
Actinomyces naeslundii | - |
- |
extracellular | - |
Actinomyces naeslundii | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | Actinomyces naeslundii | enzyme is probably involved in initiation and progression of dental caries and periodontal diseases | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | Actinomyces naeslundii | capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth | alpha-D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | Actinomyces naeslundii WVU45 | enzyme is probably involved in initiation and progression of dental caries and periodontal diseases | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | Actinomyces naeslundii WVU45 | capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth | alpha-D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomyces naeslundii | - |
- |
- |
Actinomyces naeslundii | - |
gene ftf | - |
Actinomyces naeslundii WVU45 | - |
- |
- |
Actinomyces naeslundii WVU45 | - |
gene ftf | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | - |
Actinomyces naeslundii |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from E. coli | Actinomyces naeslundii |
SDS-PAGE, immobilon-P membrane | Actinomyces naeslundii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Actinomyces naeslundii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1217 | - |
sucrose, in culture supernatants | Actinomyces naeslundii |
0.13 | - |
glucose, in culture supernatants | Actinomyces naeslundii |
2.562 | - |
glucose, in wild-type strain | Actinomyces naeslundii |
3.747 | - |
sucrose, in wild-type strain | Actinomyces naeslundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Actinomyces naeslundii | ? | - |
? | |
inulin + H2O | - |
Actinomyces naeslundii WVU45 | ? | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | reverse reaction is probably catalyzed by a different levanase | Actinomyces naeslundii | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | enzyme is probably involved in initiation and progression of dental caries and periodontal diseases | Actinomyces naeslundii | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | reverse reaction is probably catalyzed by a different levanase | Actinomyces naeslundii WVU45 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | enzyme is probably involved in initiation and progression of dental caries and periodontal diseases | Actinomyces naeslundii WVU45 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Actinomyces naeslundii | alpha-D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth | Actinomyces naeslundii | alpha-D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Actinomyces naeslundii WVU45 | alpha-D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth | Actinomyces naeslundii WVU45 | alpha-D-glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000-72000 | Actinomyces naeslundii |
Synonyms | Comment | Organism |
---|---|---|
levanase-sucrase | - |
Actinomyces naeslundii |
LevJ | - |
Actinomyces naeslundii |