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Literature summary for 2.4.1.10 extracted from

  • Hochstrasser, U.; Luscher, M.; De Virgilio, C.; Boller, T.; Wiemken, A.
    Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris (1998), FEBS Lett., 440, 356-360.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hordeum vulgare
expression in Pichia pastoris X-33 from plasmid containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor for entry into the secretory pathway, secretion into the medium Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
additional information enzyme functionally expressed in Pichia pastoris and secreted into the medium shows additional sucrose-sucrose 1-fructosyltransferase activity, methanol induction Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n Hordeum vulgare
-
glucose + (2,6-beta-D-fructosyl)n+1
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
levansucrase and additional beta-fructosidase, i.e. invertase, sucrose-sucrose 1-fructosyltransferase, and sucrose-sucrose 6-fructosyltransferase activities of recombinant enzyme from Pichia pastoris, overview Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + (2,6-beta-D-fructosyl)n additional beta-fructosidase, i.e. invertase, sucrose-sucrose 1-fructosyltransferase, and sucrose-sucrose 6-fructosyltransferase activities of recombinant enzyme from Pichia pastoris Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
?

Synonyms

Synonyms Comment Organism
6-SFT
-
Hordeum vulgare
sucrose:fructan 6-fructosyltransferase
-
Hordeum vulgare