Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | inactivation at 8 M | Actinomyces viscosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
sucrose | - |
Actinomyces viscosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | associated | Actinomyces viscosus | 5618 | - |
cell wall | cell wall associated enzyme is probably identical with the soluble extracellular one | Actinomyces viscosus | 5618 | - |
extracellular | cell wall associated enzyme is probably identical with the soluble extracellular one | Actinomyces viscosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
220000 | - |
extracellular, gel filtration | Actinomyces viscosus |
250000 | - |
cell-wall-associated enzyme form, gel filtration | Actinomyces viscosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomyces viscosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
high ionic strength salt solutions fail to remove the enzyme from the cell wall, e.g. 8 M LiCl, 2 M KCl, 6 M CsCl, 2.5 M ammonium sulfate | Actinomyces viscosus |
partial | Actinomyces viscosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
partially purified enzyme from cell wall | Actinomyces viscosus |
90 | - |
partially purified extracellular enzyme | Actinomyces viscosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | - |
Actinomyces viscosus | glucose + (2,6-beta-D-fructosyl)n+1 | high molecular weight branched levan | ? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Actinomyces viscosus | glucose + (2,6-beta-D-fructosyl)n+1 | product forms a complex with the enzyme | ? |