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Literature summary for 2.4.1.10 extracted from

  • Pabst, M.J.; Cisar, J.O.; Trummel, C.L.
    The cell wall-associated levansucrase of Actinomyces viscosus (1979), Biochim. Biophys. Acta, 566, 274-282.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Li+ inactivation at 8 M Actinomyces viscosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
sucrose
-
Actinomyces viscosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall associated Actinomyces viscosus 5618
-
cell wall cell wall associated enzyme is probably identical with the soluble extracellular one Actinomyces viscosus 5618
-
extracellular cell wall associated enzyme is probably identical with the soluble extracellular one Actinomyces viscosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
extracellular, gel filtration Actinomyces viscosus
250000
-
cell-wall-associated enzyme form, gel filtration Actinomyces viscosus

Organism

Organism UniProt Comment Textmining
Actinomyces viscosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
high ionic strength salt solutions fail to remove the enzyme from the cell wall, e.g. 8 M LiCl, 2 M KCl, 6 M CsCl, 2.5 M ammonium sulfate Actinomyces viscosus
partial Actinomyces viscosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
partially purified enzyme from cell wall Actinomyces viscosus
90
-
partially purified extracellular enzyme Actinomyces viscosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Actinomyces viscosus glucose + (2,6-beta-D-fructosyl)n+1 high molecular weight branched levan ?
sucrose + (2,6-beta-D-fructosyl)n
-
Actinomyces viscosus glucose + (2,6-beta-D-fructosyl)n+1 product forms a complex with the enzyme ?