Crystallization (Comment) | Organism |
---|---|
crystallization from saturated (NH4)2SO4-solution, pH 6.0, 4°C, 20 mM potassium phosphate, 0.2 M NaCl | Zymomonas mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Zymomonas mobilis | |
Cu2+ | - |
Zymomonas mobilis | |
Hg2+ | - |
Zymomonas mobilis | |
Mersalyl acid | - |
Zymomonas mobilis | |
additional information | not: EDTA | Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
122 | - |
sucrose | - |
Zymomonas mobilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Zymomonas mobilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Zymomonas mobilis |
94000 | - |
gel filtration | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | at lower temperatures, such as 5°C and 15°C and pH 4.0-6.0 the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C and pH 7.0-8.0 the hydrolysis is preferentially catalyzed | Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
r | |
sucrose + (2,6-beta-D-fructosyl)n | enzyme catalyzes the liberation of reducing sugars from substrates having 2-beta-D-fructofuranose residues on a terminal such as sucrose, raffinose, levan from Aerobacter levanicum and inulin with a relative activity of 100:104:1:0.01 | Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
r | |
sucrose + H2O | hydrolysis | Zymomonas mobilis | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, SDS-PAGE | Zymomonas mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
at lower temperatures, such as 4°C and 15°C, the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C the hydrolysis is preferentially catalyzed | Zymomonas mobilis |
30 | - |
assay at | Zymomonas mobilis |
50 | - |
- |
Zymomonas mobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 6.0, 30 min | Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | - |
Zymomonas mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
15°C, 10% sucrose, pH 4.0-6.0: transfructosylation preferentially occurs and oligo- and polysaccharides are produced, pH 7.0-8.0: hydrolysis of sucrose is enhanced, oligosaccharides are produced rather than polysaccharides as transfructosylation products | Zymomonas mobilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | 30°C, 24 h, stable | Zymomonas mobilis |