Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | quantification of the allosteric effect and the thermodynamic effect of AMP on phosphorylase b, overview | Oryctolagus cuniculus | |
sulfate | activates the wild-type but not the N-terminally truncated mutant | Oryctolagus cuniculus |
Application | Comment | Organism |
---|---|---|
drug development | glycogen phosphorylase is an active target of research in search of inhibitors for the treatment of type II diabetes | Oryctolagus cuniculus |
Cloned (Comment) | Organism |
---|---|
expression of Pho1 in Escherichia coli strain DF1020 | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an N-terminal truncated mutant DELTA2-17 | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | allosteric kinetics, overview | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate | Oryctolagus cuniculus | - |
[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Oryctolagus cuniculus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
glycogen phosphorylase b | - |
Oryctolagus cuniculus |
phosphorylase a | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Oryctolagus cuniculus |
General Information | Comment | Organism |
---|---|---|
additional information | phosphorylase a is produced and isolated utilizing purified phosphorylase b and commercially available phosphorylase kinase | Oryctolagus cuniculus |