Any feedback?
Literature summary for 2.4.1.1 extracted from
- Sweet corn phosphorylase: purification and properties (1973), Arch. Biochem. Biophys., 156, 276-286.
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 10%, stabilizes during purification | Zea mays |
| sucrose, 10%, stabilizes during purification | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | 5 mM, 30% inhibition | Zea mays |  |
| ADPglucose | 5 mM, 30% inhibition | Zea mays | |
| additional information | not inhibited by GMP; not inhibited by GTP; not inhibited by UMP, CMP, CTP; not inhibited by fructose 6-phosphate | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data | Zea mays |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
sucrose density gradient ultracentrifugation | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
sweet corn, var. Golden Bantam, Iowa Belle 104 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, hydroxylapatite | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35 | 49 | - |
Zea mays |
| 49 | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (maltodextrin)n-1 + glucose 1-phosphate | - |
Zea mays | (maltodextrin)n + phosphate | - |
r | |
| amylopectin + glucose 1-phosphate | best substrate | Zea mays | amylopectin + phosphate | - |
? | |
| glycogen + glucose 1-phosphate | poor substrate | Zea mays | glycogen + phosphate | - |
? | |
| maltoheptaose + glucose 1-phosphate | - |
Zea mays | maltooctaose + phosphate | - |
r | |
| maltohexaose + glucose 1-phosphate | - |
Zea mays | maltoheptaose + phosphate | - |
r | |
| maltooctaose + glucose 1-phosphate | - |
Zea mays | maltononaose + phosphate | - |
r | |
| maltopentaose + glucose 1-phosphate | - |
Zea mays | maltohexaose + phosphate | - |
r | |
| maltotetraose + glucose 1-phosphate | - |
Zea mays | maltopentaose + phosphate | - |
ir | |
| maltotriose + glucose 1-phosphate | - |
Zea mays | maltotetraose + phosphate | - |
ir | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | polyglucose primer required | Zea mays | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
shoulder at pH 6.5 | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | requirement | Zea mays | |
| pyridoxal 5'-phosphate | 1 mol/mol subunit | Zea mays | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
