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Literature summary for 2.4.1.1 extracted from
- Characterization of the spinach leaf phosphorylases (1980), Plant Physiol., 66, 864-869.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
phosphate | cytoplasmic phosphorylase, pH 7.0 | Spinacia oleracea |  |
| 1.2 | - |
phosphate | chloroplast phosphorylase, pH 7.0 | Spinacia oleracea | |
| 1.5 | - |
phosphate | cytoplasmic phosphorylase, pH 8.0 | Spinacia oleracea | |
| 1.8 | - |
phosphate | chloroplastphosphorylase, pH 8.0 | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| cytoplasm | - |
Spinacia oleracea | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
2 * 92000, cytoplasmic isozyme, SDS-PAGE | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cytoplasmic phosphorylase: ammonium sulfate, DEAE-cellulose, glycogen-Sepharose, chloroplast phosphorylase: DEAE-cellulose, Biogel A, partial purification | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
chloroplastic enzyme, phosphorolysis | Spinacia oleracea |
| 43.5 | - |
cytoplasmic enzyme, synthesis | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + glucose 1-phosphate | effective primer | Spinacia oleracea | amylopectin + phosphate | - |
r | |
| amylose + glucose 1-phosphate | effective primer | Spinacia oleracea | amylose + phosphate | - |
r | |
| amylose + glucose 1-phosphate | potato amylose | Spinacia oleracea | amylose + phosphate | - |
r | |
| debranched amylopectin + glucose 1-phosphate | most effective primer glucan for chloroplastic enzyme | Spinacia oleracea | debranched amylopectin + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | glycogen from: rabbit liver | Spinacia oleracea | glycogen + phosphate | - |
r | |
| maltopentaose + glucose 1-phosphate | cytoplasmic enzyme, glucosylation at the same rate of glucosylation of debranched amylopectin, chloroplastic enzyme, glucosylation at 50% the rate of glucosylation of debranched amylopectin | Spinacia oleracea | maltohexaose + phosphate | - |
r | |
| maltotetraose + glucose 1-phosphate | cytoplasmic enzyme: poor substrate | Spinacia oleracea | maltopentaose + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | polyglucose primer required | Spinacia oleracea | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 92000, cytoplasmic isozyme, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | cytoplasmic enzyme, buffer dependent optima in this range | Spinacia oleracea |
| 7.5 | - |
broad optimum, chloroplast enzyme, phosphorolysis | Spinacia oleracea |
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