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Literature summary for 2.4.1.1 extracted from

  • Nakamura, Y.; Imamura, M.
    Characterization of Chlorella phosphorylase: the glucan specificity and effect of temperature (1983), Phytochemistry, 22, 2395-2399.
No PubMed abstract available
Search for more literature for 2.4.1.1

Inhibitors

Inhibitors Comment Organism Structure
ADPglucose kinetics Chlorella vulgaris
additional information not inhibited by maltose, maltotriose, maltotetraose Chlorella vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Chlorella vulgaris
additional information
-
 additional information amylopectin, 0.69 mg/ml and 0.68 mg/ml for phosphorolysis and synthesis, respectively Chlorella vulgaris
0.21
-
 maltodecaheptaose phosphorolysis Chlorella vulgaris
0.5
-
 maltodecaheptaose synthesis Chlorella vulgaris
0.68
-
 maltohexaose phosphorolysis Chlorella vulgaris
0.72
-
 maltooctaose phosphorolysis Chlorella vulgaris
0.93
-
 maltopentaose phosphorolysis Chlorella vulgaris
1.22
-
 maltoheptaose phosphorolysis Chlorella vulgaris
2
-
 maltooctaose synthesis Chlorella vulgaris
2 8.3 maltooctaose maltoheptaose, maltohexaose, maltopentaose, maltotetraose, synthesis Chlorella vulgaris
2.2
-
 maltoheptaose synthesis Chlorella vulgaris
2.5
-
 maltohexaose synthesis Chlorella vulgaris
3
-
 maltopentaose synthesis Chlorella vulgaris
8.3
-
 maltotetraose synthesis Chlorella vulgaris

Organism

Organism UniProt Comment Textmining
Chlorella vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Chlorella vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(maltodextrin)n-1 + glucose 1-phosphate phosphorolysis activity ceases when maltodextrins are degraded to maltotetraose Chlorella vulgaris (maltodextrin)n + phosphate
-
 r
amylopectin + glucose 1-phosphate best substrate Chlorella vulgaris amylopectin + phosphate
-
 r
glycogen + glucose 1-phosphate rabbit liver glycogen Chlorella vulgaris glycogen + phosphate
-
 r
glycogen + glucose 1-phosphate glycogen from oyster Chlorella vulgaris glycogen + phosphate
-
 r
glycogen + glucose 1-phosphate glycogen from: rabbit liver Chlorella vulgaris glycogen + phosphate
-
 r
maltodecaheptaose + phosphate
-
 Chlorella vulgaris maltodecahexaose + alpha-D-glucose 1-phosphate
-
 r
maltoheptadecaose + glucose 1-phosphate
-
 Chlorella vulgaris maltooctadecaose + phosphate
-
 r
maltoheptaose + glucose 1-phosphate
-
 Chlorella vulgaris maltooctaose + phosphate
-
 r
maltohexaose + glucose 1-phosphate
-
 Chlorella vulgaris maltoheptaose + phosphate
-
 r
maltooctaose + glucose 1-phosphate
-
 Chlorella vulgaris maltononaose + phosphate
-
 r
maltopentaose + glucose 1-phosphate
-
 Chlorella vulgaris maltohexaose + phosphate
-
 r
maltotetraose + glucose 1-phosphate best substrate Chlorella vulgaris maltopentaose + phosphate
-
 ir
[(1->4)-alpha-D-glucosyl]n + phosphate ineffective primer: glucose Chlorella vulgaris [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate polyglucose primer required Chlorella vulgaris [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 ratio of phosphorolysis to synthesis increases significantly at higher temperatures Chlorella vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1 6.8 synthesis, 2-(N-morpholino)ethanesulfonic acid buffer Chlorella vulgaris
7 7.5 phosphorolysis Chlorella vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
5.6 7.5 approx. half-maximal activity at pH 5.6 and pH 7.5, imidazole buffer, phosphorolysis Chlorella vulgaris
7 8.1 maximal activity at pH 7, approx. 60% of maximal activity at pH 8.1, glycylglycine buffer, phosphorolysis Chlorella vulgaris