Literature summary for 2.4.1.1 extracted from

Nader, W.; Becker, J.U.
1,4-alpha-Glucan phosphorylase from the slime mold Physarum polycephalum. Purification, physico-chemical and kinetic properties (1979), Eur. J. Biochem., 102, 345-355.

Search for more literature for 2.4.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
5'-AMP	activation	Physarum polycephalum
5'-AMP	slight activation	Physarum polycephalum

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucose	weak	Physarum polycephalum
fructose 1-phosphate	-	Physarum polycephalum
fructose 6-phosphate	-	Physarum polycephalum
GDPglucose	kinetics	Physarum polycephalum
glucose 1-phosphate	completely reversible by 5'-AMP; kinetics	Physarum polycephalum
glucose 6-phosphate	-	Physarum polycephalum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetic data	Physarum polycephalum
additional information	-	additional information	1.26 mg/ml, glycogen	Physarum polycephalum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
93000	-	2 * 93000, SDS-PAGE	Physarum polycephalum
172000	-	gel filtration	Physarum polycephalum

Organism

Organism	UniProt	Comment	Textmining
Physarum polycephalum	-	M3c	-
Physarum polycephalum M3c	-	M3c	-

Purification (Commentary)

Purification (Comment)	Organism
Streptomycin, ammonium sulfate, DEAE-cellulose, aminobutyl-Sepharose, hydroxyapatite	Physarum polycephalum

Source Tissue

Source Tissue	Comment	Organism	Textmining
plasmodium	macroplasmodium	Physarum polycephalum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.2	-	-	Physarum polycephalum

Storage Stability

Storage Stability	Organism
6°C, 3 months	Physarum polycephalum
bovine serum albumin prevents surface inactivation during storage	Physarum polycephalum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycogen + glucose 1-phosphate	glycogen from oyster	Physarum polycephalum	glycogen + phosphate	-	r
glycogen + glucose 1-phosphate	glycogen from Streptococcus salivarius	Physarum polycephalum	glycogen + phosphate	-	r
glycogen + glucose 1-phosphate	glycogen from oyster	Physarum polycephalum M3c	glycogen + phosphate	-	r
glycogen + glucose 1-phosphate	glycogen from Streptococcus salivarius	Physarum polycephalum M3c	glycogen + phosphate	-	r
[(1->4)-alpha-D-glucosyl]n + phosphate	-	Physarum polycephalum	[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate	-	r
[(1->4)-alpha-D-glucosyl]n + phosphate	-	Physarum polycephalum M3c	[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 93000, SDS-PAGE	Physarum polycephalum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	7.5	broad	Physarum polycephalum

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.4	8.2	approx. half-maximal activity at pH 5.4 and pH 8.2	Physarum polycephalum

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	requirement	Physarum polycephalum
pyridoxal 5'-phosphate	1 mol/mol subunit	Physarum polycephalum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.03	-	GDP-glucose	-	Physarum polycephalum
0.1	-	glucose 1-phosphate	-	Physarum polycephalum

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Release 2023.1 (January 2023)