Any feedback?
Literature summary for 2.3.3.9 extracted from
- Biochemical characterization of malate synthase G of P. aeruginosa (2009), BMC Biochem., 10, 20.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GST-tagged version expressed in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pyruvate | - |
Pseudomonas aeruginosa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
acetyl-CoA | - |
Pseudomonas aeruginosa | |
| 0.07 | - |
glyoxylate | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79400 | - |
x * 79400, predicted and observed (SDS-PAGE) | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione affinity chromatography, proteolytic removal of the GST tag, anion exchange chromatography | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.5 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glyoxylate + H2O | - |
Pseudomonas aeruginosa | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 79400, predicted and observed (SDS-PAGE) | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malate synthase G | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37.5 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
15.5% activity | Pseudomonas aeruginosa |
| 15 | - |
47.1% activity | Pseudomonas aeruginosa |
| 32.5 | 40 | more than 90% of activity | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1 h preincubation: 100% activity | Pseudomonas aeruginosa |
| 55 | - |
1 h preincubation: 47% activity | Pseudomonas aeruginosa |
| 70 | - |
1 h preincubation: complete loss of activity | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
below pH 6: no activity remains, outside pH 7-10 the activity decreases rapidly | Pseudomonas aeruginosa |
| 7 | - |
87% activity | Pseudomonas aeruginosa |
| 10 | - |
85% activity | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 2 | 1 h preincubation: about 5% activity | Pseudomonas aeruginosa |
| 5 | - |
1 h preincubation: about 5% activity | Pseudomonas aeruginosa |
| 6 | 7 | 1 h preincubation: 100% activity | Pseudomonas aeruginosa |
| 11 | - |
1 h preincubation: 90% activity | Pseudomonas aeruginosa |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
1 mM pyruvate: 40% activity | Pseudomonas aeruginosa | pyruvate | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
