Cloned (Comment) | Organism |
---|---|
- |
Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | bifunctional enzyme has specific catalytic features. Isocitrate lyase activity is increased by acetyl-CoA | Euglena gracilis | |
0.025 | - |
acetyl-CoA | 30°C, pH 8.0 | Euglena gracilis | |
0.04 | - |
glyoxylate | 30°C, pH 8.0 | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
4 * 110000, SDS-PAGE | Euglena gracilis |
420000 | - |
gel filtration | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | Q8LPA6 | bifunctional malate synthase/isocitrate lyase, induced by growth on ethanol | - |
Purification (Comment) | Organism |
---|---|
- |
Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.19 | - |
30°C, pH 8.0 | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glyoxylate + H2O | - |
Euglena gracilis | (S)-malate + CoA | - |
? | |
acetyl-CoA + glyoxylate + H2O | - |
Euglena gracilis SM-ZK | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme consists of N-terminal malate synthase domain fused to C-terminal isocitrate lyase domain | Euglena gracilis |
tetramer | 4 * 110000, SDS-PAGE | Euglena gracilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | malate synthase reaction | Euglena gracilis |