General Stability | Organism |
---|---|
freezing and thawing inactivates | Pseudomonas putida |
Mg2+ stabilizes | Saccharomyces cerevisiae |
Mg2+ stabilizes | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoroacetate | - |
Pseudomonas putida | |
fluoroacetate | - |
Saccharomyces cerevisiae | |
glycolate | - |
Pseudomonas putida | |
glycolate | - |
Saccharomyces cerevisiae | |
oxalate | - |
Pseudomonas putida | |
oxalate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
glyoxylate | - |
Pseudomonas putida | |
0.093 | - |
glyoxylate | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae | |
Mg2+ | required | Pseudomonas putida | |
Mg2+ | Km: 0.5 mM | Saccharomyces cerevisiae | |
Mg2+ | Km: 0.5 mM | Pseudomonas putida | |
Mn2+ | can partially replace Mg2+ in activation | Saccharomyces cerevisiae | |
Mn2+ | can partially replace Mg2+ in activation | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
2°C, stable for at least a month | Saccharomyces cerevisiae |
2°C, stable for at least a month | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-malate + CoA | - |
ir | |
glyoxylate + acetyl-CoA + H2O | - |
Pseudomonas putida | (S)-malate + CoA | - |
ir | |
glyoxylate + fluoroacetyl-CoA + H2O | - |
Pseudomonas putida | 2-oxo-3-fluoro-butanedioic acid + CoA | - |
ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
additional information | - |
additional information | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Saccharomyces cerevisiae |
8.5 | - |
- |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
oxalate | - |
Saccharomyces cerevisiae | |
0.019 | - |
oxalate | - |
Pseudomonas putida | |
0.246 | - |
fluoroacetate | - |
Saccharomyces cerevisiae | |
0.246 | - |
fluoroacetate | - |
Pseudomonas putida | |
0.308 | - |
glycolate | - |
Saccharomyces cerevisiae | |
0.308 | - |
glycolate | - |
Pseudomonas putida |