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Literature summary for 2.3.3.8 extracted from

  • Hu, J.; Komakula, A.; Fraser, M.E.
    Binding of hydroxycitrate to human ATP-citrate lyase (2017), Acta Crystallogr. Sect. D, 73, 660-671 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour diffusion in hanging drops. The protein is modified by introducing cleavage sites for Tobacco etch virus protease on either side of a disordered linker. The protein crystallized consists of residues 2-425-ENLYFQ and S-488-810 of human ATP-citrate lyase. When co-crystals are grown with ATP and magnesium ions as well as either the inhibitor (2S,3S)-2-hydroxycitrate or citrate, Mg2+-ADP is bound and His760 is phosphorylated Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4S-hydroxycitrate
-
Homo sapiens
ADP
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0308
-
citrate pH and temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + citrate + CoA Homo sapiens acetyl-CoA is produced in mitochondria, but it cannot cross the mitochondrial membranes to the cytosol. Instead, citrate is exported from mitochondria. In the cytosol, ATP-citrate lyase (ACLY; EC 2.3.3.8) catalyses the reaction to produce acetyl-CoA and oxaloacetate from citrate and ATP ADP + phosphate + acetyl-CoA + oxaloacetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P53396
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrate + CoA
-
Homo sapiens ADP + phosphate + acetyl-CoA + oxaloacetate
-
?
ATP + citrate + CoA acetyl-CoA is produced in mitochondria, but it cannot cross the mitochondrial membranes to the cytosol. Instead, citrate is exported from mitochondria. In the cytosol, ATP-citrate lyase (ACLY; EC 2.3.3.8) catalyses the reaction to produce acetyl-CoA and oxaloacetate from citrate and ATP Homo sapiens ADP + phosphate + acetyl-CoA + oxaloacetate
-
?

Synonyms

Synonyms Comment Organism
ACLY
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.137
-
ADP pH and temperature not specified in the publication Homo sapiens