Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion in hanging drops. The protein is modified by introducing cleavage sites for Tobacco etch virus protease on either side of a disordered linker. The protein crystallized consists of residues 2-425-ENLYFQ and S-488-810 of human ATP-citrate lyase. When co-crystals are grown with ATP and magnesium ions as well as either the inhibitor (2S,3S)-2-hydroxycitrate or citrate, Mg2+-ADP is bound and His760 is phosphorylated | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4S-hydroxycitrate | - |
Homo sapiens | |
ADP | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0308 | - |
citrate | pH and temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + CoA | Homo sapiens | acetyl-CoA is produced in mitochondria, but it cannot cross the mitochondrial membranes to the cytosol. Instead, citrate is exported from mitochondria. In the cytosol, ATP-citrate lyase (ACLY; EC 2.3.3.8) catalyses the reaction to produce acetyl-CoA and oxaloacetate from citrate and ATP | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53396 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + CoA | - |
Homo sapiens | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
ATP + citrate + CoA | acetyl-CoA is produced in mitochondria, but it cannot cross the mitochondrial membranes to the cytosol. Instead, citrate is exported from mitochondria. In the cytosol, ATP-citrate lyase (ACLY; EC 2.3.3.8) catalyses the reaction to produce acetyl-CoA and oxaloacetate from citrate and ATP | Homo sapiens | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACLY | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.137 | - |
ADP | pH and temperature not specified in the publication | Homo sapiens |