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Literature summary for 2.3.3.8 extracted from

  • Chavez-Cabrera, C.; Marsch, R.; Bartolo-Aguilar, Y.; Flores-Bustamante, Z.R.; Hidalgo-Lara, M.E.; Martinez-Cardenas, A.; Cancino-Diaz, J.C.; Sanchez, S.; Flores-Cotera, L.B.
    Molecular cloning and characterization of the ATP citrate lyase from carotenogenic yeast Phaffia rhodozyma (2015), FEMS Yeast Res., 15, fov054.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Phaffia rhodozyma

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Phaffia rhodozyma 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
4 * 120000, MALDI TOF mass spectrometry Phaffia rhodozyma
120100
-
4 * 120100, calculated from amino acid sequence Phaffia rhodozyma

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphate + acetyl-CoA + oxaloacetate Phaffia rhodozyma
-
ATP + citrate + CoA
-
?
ADP + phosphate + acetyl-CoA + oxaloacetate Phaffia rhodozyma NRRL Y-10922
-
ATP + citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Phaffia rhodozyma A0A0K0NLA0
-
-
Phaffia rhodozyma NRRL Y-10922 A0A0K0NLA0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and gel filtration Phaffia rhodozyma

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.036
-
crude enzyme, at pH 8.5, temperature not specified in the publication Phaffia rhodozyma
2
-
after 56fold purification, at pH 8.5, temperature not specified in the publication Phaffia rhodozyma

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + acetyl-CoA + oxaloacetate
-
Phaffia rhodozyma ATP + citrate + CoA
-
?
ADP + phosphate + acetyl-CoA + oxaloacetate
-
Phaffia rhodozyma NRRL Y-10922 ATP + citrate + CoA
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 120000, MALDI TOF mass spectrometry Phaffia rhodozyma
homotetramer 4 * 120100, calculated from amino acid sequence Phaffia rhodozyma

Synonyms

Synonyms Comment Organism
ACL
-
Phaffia rhodozyma
ATP citrate lyase
-
Phaffia rhodozyma

pI Value

Organism Comment pI Value Maximum pI Value
Phaffia rhodozyma calculated from amino acid sequence
-
8.4