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Literature summary for 2.3.3.8 extracted from

  • Sun, T.; Hayakawa, K.; Bateman, K.S.; Fraser, M.E.
    Identification of the citrate-binding site of human ATP-citrate lyase using X-ray crystallography (2010), J. Biol. Chem., 285, 27418-27428.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with citrate or tartrate, hanging drop vapor diffusion method, using either 12.5% (w/v) PEG 3350, 100 mM sodium tartrate, 100 mM Tris-HCl (pH 7.0) for the native protein or 10% PEG 3350, 75 mM potassium citrate, 100 mM Tris-HCl (pH 7.0) for the selenomethionyl protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Tartrate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P53396
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, Q-Sepharose column chromatography and DEAE-Sephacel gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrate + CoA
-
 Homo sapiens ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ?

Synonyms

Synonyms Comment Organism
ACLY
-
 Homo sapiens
ATP-citrate lyase
-
 Homo sapiens