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Literature summary for 2.3.3.8 extracted from
- ATP-citrate lyase from the green sulfur bacterium Chlorobium limicola is a heteromeric enzyme composed of two distinct gene products (2001), Eur. J. Biochem., 268, 1670-1678.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Chlorobium limicola |  |
| oxaloacetate | - |
Chlorobium limicola | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Chlorobium limicola | |
| Mn2+ | may substitue for Mg2+ | Chlorobium limicola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 43675 + y * 65535, calculated, x * 40000 + x * 65000, SDS-PAGE | Chlorobium limicola |
| 43675 | - |
x * 43675 + y * 65535, calculated, x * 40000 + x * 65000, SDS-PAGE | Chlorobium limicola |
| 65000 | - |
x * 43675 + y * 65535, calculated, x * 40000 + x * 65000, SDS-PAGE | Chlorobium limicola |
| 65535 | - |
x * 43675 + y * 65535, calculated, x * 40000 + x * 65000, SDS-PAGE | Chlorobium limicola |
| 532000 | 557000 | gel filtration | Chlorobium limicola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorobium limicola | Q9AQH6 | gene acLB, beta-subunit; subunit ORF1, product of gene acLB, both subunits ORF1 and ORF2 are essential for enzyme activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification from Chlorobium limicola, complete purification after expression in Escherichia coli | Chlorobium limicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + citrate + CoA | - |
Chlorobium limicola | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
| additional information | dATP may substitute for ATP, no substrate: malate, isocitrate, succinate, acetate | Chlorobium limicola | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43675 + y * 65535, calculated, x * 40000 + x * 65000, SDS-PAGE | Chlorobium limicola |
| More | N-terminal amino acid sequence | Chlorobium limicola |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Chlorobium limicola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | maximum activity | Chlorobium limicola |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | more than 80% of maximum activity within this range | Chlorobium limicola |
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Release 2023.1 (January 2023)
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