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Literature summary for 2.3.3.8 extracted from
- ATP:citrate lyase of Rhodotorula gracilis: purification and properties (1990), Biochim. Biophys. Acta, 1033, 23-30.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | stimulates | Rhodotorula toruloides |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 1 mM | Rhodotorula toruloides | |
| AMP | 1 mM | Rhodotorula toruloides | |
| D-glucose 6-phosphate | - |
Rhodotorula toruloides | |
| Glu | - |
Rhodotorula toruloides | |
| lauroyl-CoA | - |
Rhodotorula toruloides | |
| myristoyl-CoA | - |
Rhodotorula toruloides | |
| oleoyl-CoA | - |
Rhodotorula toruloides | |
| palmitoyl-CoA | - |
Rhodotorula toruloides | |
| stearoyl-CoA | - |
Rhodotorula toruloides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.175 | - |
ATP | - |
Rhodotorula toruloides | |
| 1.25 | - |
citrate | 0.2 M Tris-HCl buffer containing 0.25 M KCl | Rhodotorula toruloides | |
| 2.63 | - |
citrate | in 0.1 M Tris-HCl buffer | Rhodotorula toruloides | |
| 3.44 | - |
citrate | in Tris-acetate buffer | Rhodotorula toruloides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
4 * 120000, the two minor proteins 51000 Da and 49000 Da can be the result of proteolytic cleavage of ATP:citrate lyase by an endogenous trypsin-like proteinase | Rhodotorula toruloides |
| 520000 | - |
gel filtration | Rhodotorula toruloides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula toruloides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodotorula toruloides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% glycerol, stable up to 2 months | Rhodotorula toruloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + citrate + CoA | - |
Rhodotorula toruloides | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 120000, the two minor proteins 51000 Da and 49000 Da can be the result of proteolytic cleavage of ATP:citrate lyase by an endogenous trypsin-like proteinase | Rhodotorula toruloides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Rhodotorula toruloides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Rhodotorula toruloides |
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Release 2023.1 (January 2023)
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