Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Hydrogenobacter thermophilus | |
dATP | - |
Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0408 | - |
CoA | - |
Hydrogenobacter thermophilus | |
0.65 | - |
ATP | - |
Hydrogenobacter thermophilus | |
6.25 | - |
citrate | - |
Hydrogenobacter thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mn2+ or Mg2+ required | Hydrogenobacter thermophilus | |
Mg2+ | Km: 8 mM MgCl2 | Hydrogenobacter thermophilus | |
Mn2+ | Mn2+ or Mg2+ required | Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
6 * 43000, SDS-PAGE | Hydrogenobacter thermophilus |
260000 | - |
gel filtration | Hydrogenobacter thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | - |
- |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.7 | - |
- |
Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + acetyl-CoA + oxaloacetate | - |
Hydrogenobacter thermophilus | ATP + citrate + CoA | - |
r | |
ADP + phosphate + acetyl-CoA + oxaloacetate | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | ATP + citrate + CoA | - |
r | |
ATP + citrate + CoA | the synthase activity is less than 0.5% of the ATP:citrate lyase activity | Hydrogenobacter thermophilus | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
r | |
ATP + citrate + CoA | the synthase activity is less than 0.5% of the ATP:citrate lyase activity | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
r | |
dATP + citrate + CoA | - |
Hydrogenobacter thermophilus | dADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
dATP + citrate + CoA | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | dADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
GTP + citrate + CoA | - |
Hydrogenobacter thermophilus | GDP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
GTP + citrate + CoA | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | GDP + phosphate + acetyl-CoA + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 43000, SDS-PAGE | Hydrogenobacter thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Hydrogenobacter thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable up to | Hydrogenobacter thermophilus |
95 | - |
30 min, more than 90% loss of activity in absence of citrate, less than 10% loss of activity in presence of citrate | Hydrogenobacter thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 6.9 | - |
Hydrogenobacter thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | stable | Hydrogenobacter thermophilus |