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Literature summary for 2.3.3.5 extracted from
- Cloning, expression, purification and bioinformatic analysis of 2-methylcitrate synthase from Mycobacterium tuberculosis (2015), Asian Pac. J. Trop. Med., 8, 19-23.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Mycobacterium tuberculosis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42900 | - |
x * 42900, calculated from amino acid sequence | Mycobacterium tuberculosis |
| 43000 | - |
x * 43000, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propanoyl-CoA + H2O + oxaloacetate | Mycobacterium tuberculosis | - |
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? |  |
| propanoyl-CoA + H2O + oxaloacetate | Mycobacterium tuberculosis H37Rv | - |
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propanoyl-CoA + H2O + oxaloacetate | - |
Mycobacterium tuberculosis | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
| propanoyl-CoA + H2O + oxaloacetate | - |
Mycobacterium tuberculosis H37Rv | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Mycobacterium tuberculosis |
| ? | x * 42900, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Rv1131 | - |
Mycobacterium tuberculosis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mycobacterium tuberculosis | calculated from amino acid sequence | - |
8.6 |
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