Cloned (Comment) | Organism |
---|---|
gene mscA, DNA and amino acid sequence determination and analysis, overexpression in Aspergillus nidulans | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme deletion mutant strain, which is unable to grow on propionate, phenotype and growth behaviour, overview | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 30% inhibition at 3 mM | Aspergillus nidulans | |
propionate | - |
Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00063 | - |
oxaloacetate | pH 8.0, 25°C, purified native enzyme | Aspergillus nidulans | |
0.0017 | - |
propanoyl-CoA | pH 8.0, 25°C, purified native enzyme | Aspergillus nidulans | |
0.0025 | - |
acetyl-CoA | pH 8.0, 25°C, purified native enzyme | Aspergillus nidulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial matrix | - |
Aspergillus nidulans | 5759 | - |
mitochondrion | enzyme possesses a mitochondrial signal import sequence | Aspergillus nidulans | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+, Cu2+, Ni2+, Co2+, and EDTA do not have any effect on the enzyme | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation | Aspergillus nidulans |
50580 | - |
2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation | Aspergillus nidulans |
90000 | - |
native enzyme, gel filtration | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propanoyl-CoA + H2O + oxaloacetate | Aspergillus nidulans | reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview | (2S,3S)-2-methylcitrate + CoA | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
strains SMI45 and SMB/acuA, the latter is acetyl-CoA synthase-deficient, gene mscA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | N-terminal processing of the mitochondrial signal import sequence | Aspergillus nidulans |
Purification (Comment) | Organism |
---|---|
native enzyme 72.5fold from strain SMB/acuA | Aspergillus nidulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypha | - |
Aspergillus nidulans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
strain SMI45 | Aspergillus nidulans |
0.2 | - |
strain SMB/acuA | Aspergillus nidulans |
14.5 | - |
purified native enzyme, with oxaloacetate and propionyl-CoA | Aspergillus nidulans |
41.5 | - |
purified native enzyme, with oxaloacetate and acetyl-CoA | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | the enzyme also performs the citrate synthase reaction, EC 2.3.3.1 | Aspergillus nidulans | citrate + CoA | - |
ir | |
propanoyl-CoA + H2O + oxaloacetate | reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview | Aspergillus nidulans | (2S,3S)-2-methylcitrate + CoA | - |
ir | |
propanoyl-CoA + H2O + oxaloacetate | reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate | Aspergillus nidulans | (2S,3S)-2-methylcitrate + CoA | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation | Aspergillus nidulans |
Synonyms | Comment | Organism |
---|---|---|
Methylcitrate synthase | - |
Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 52 | - |
Aspergillus nidulans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Aspergillus nidulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
oxaloacetate | pH 8.0, 25°C, purified native enzyme | Aspergillus nidulans | |
23 | - |
propanoyl-CoA | pH 8.0, 25°C, purified native enzyme | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Aspergillus nidulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | - |
Aspergillus nidulans |