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Literature summary for 2.3.3.5 extracted from

  • Brock, M.; Fischer, R.; Linder, D.; Buckel, W.
    Methylcitrate synthase from Aspergillus nidulans: implications for propionate as an antifungal agent (2000), Mol. Microbiol., 35, 961-973.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mscA, DNA and amino acid sequence determination and analysis, overexpression in Aspergillus nidulans Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme deletion mutant strain, which is unable to grow on propionate, phenotype and growth behaviour, overview Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
ATP 30% inhibition at 3 mM Aspergillus nidulans
propionate
-
Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00063
-
oxaloacetate pH 8.0, 25°C, purified native enzyme Aspergillus nidulans
0.0017
-
propanoyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus nidulans
0.0025
-
acetyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial matrix
-
Aspergillus nidulans 5759
-
mitochondrion enzyme possesses a mitochondrial signal import sequence Aspergillus nidulans 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Cu2+, Ni2+, Co2+, and EDTA do not have any effect on the enzyme Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation Aspergillus nidulans
50580
-
2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation Aspergillus nidulans
90000
-
native enzyme, gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propanoyl-CoA + H2O + oxaloacetate Aspergillus nidulans reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview (2S,3S)-2-methylcitrate + CoA
-
ir

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
strains SMI45 and SMB/acuA, the latter is acetyl-CoA synthase-deficient, gene mscA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification N-terminal processing of the mitochondrial signal import sequence Aspergillus nidulans

Purification (Commentary)

Purification (Comment) Organism
native enzyme 72.5fold from strain SMB/acuA Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining
hypha
-
Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
strain SMI45 Aspergillus nidulans
0.2
-
strain SMB/acuA Aspergillus nidulans
14.5
-
purified native enzyme, with oxaloacetate and propionyl-CoA Aspergillus nidulans
41.5
-
purified native enzyme, with oxaloacetate and acetyl-CoA Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate the enzyme also performs the citrate synthase reaction, EC 2.3.3.1 Aspergillus nidulans citrate + CoA
-
ir
propanoyl-CoA + H2O + oxaloacetate reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview Aspergillus nidulans (2S,3S)-2-methylcitrate + CoA
-
ir
propanoyl-CoA + H2O + oxaloacetate reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate Aspergillus nidulans (2S,3S)-2-methylcitrate + CoA
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation Aspergillus nidulans

Synonyms

Synonyms Comment Organism
Methylcitrate synthase
-
Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 52
-
Aspergillus nidulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70
-
Aspergillus nidulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
oxaloacetate pH 8.0, 25°C, purified native enzyme Aspergillus nidulans
23
-
propanoyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
Aspergillus nidulans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10.5
-
Aspergillus nidulans