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Literature summary for 2.3.3.3 extracted from

  • Marco-Urrea, E.; Paul, S.; Khodaverdi, V.; Seifert, J.; von Bergen, M.; Kretzschmar, U.; Adrian, L.
    Identification and characterization of a Re-citrate synthase in Dehalococcoides strain CBDB1 (2011), J. Bacteriol., 193, 5171-5178.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.3

Activating Compound

Activating Compound Comment Organism Structure
AMP about 120% activity at 0.15 mM Dehalococcoides sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Dehalococcoides sp.

Inhibitors

Inhibitors Comment Organism Structure
ATP about 40% residual activity at 5 mM Dehalococcoides sp.
citrate about 40% residual activity at 10 mM Dehalococcoides sp.
L-lysine about 80% residual activity at 0.5 mM Dehalococcoides sp.
additional information not influenced by 2-oxoglutarate Dehalococcoides sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.2 mM Mn2+ is required for full activity Dehalococcoides sp.

Organism

Organism UniProt Comment Textmining
Dehalococcoides sp. P0DO96 designated as EC 2.3.3.14 but does not have any homocitrate synthase activity
-
Dehalococcoides sp. CBDB1 P0DO96 designated as EC 2.3.3.14 but does not have any homocitrate synthase activity
-

Purification (Commentary)

Purification (Comment) Organism
Strep tag affinity column chromatography Dehalococcoides sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
 Dehalococcoides sp. citrate + CoA
-
 ?
acetyl-CoA + H2O + oxaloacetate
-
 Dehalococcoides sp. CBDB1 citrate + CoA
-
 ?
additional information the protein does not have homocitrate synthase activity Dehalococcoides sp. ?
-
 ?
additional information the protein does not have homocitrate synthase activity Dehalococcoides sp. CBDB1 ?
-
 ?

Synonyms

Synonyms Comment Organism
cbdbA1708
-
 Dehalococcoides sp.
Re-citrate synthase
-
 Dehalococcoides sp.
Re-CS
-
 Dehalococcoides sp.