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Literature summary for 2.3.3.20 extracted from

  • Goblirsch, B.; Jensen, M.; Mohamed, F.; Wackett, L.; Wilmot, C.
    Substrate trapping in crystals of the thiolase Olea identifies three channels that enable long chain olefin biosynthesis (2016), J. Biol. Chem., 291, 26698-26706 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.20

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 15-20% (w/v) PEG 8000, 70-100 mM potassium phosphate dibasic, and 100 mM sodium citrate, pH 4.2 Xanthomonas campestris pv. campestris

Protein Variants

Protein Variants Comment Organism
C143A the mutant shows less than 0.1% of wild type activity Xanthomonas campestris pv. campestris
C143S the mutant shows 0.3% of wild type activity Xanthomonas campestris pv. campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an acyl-CoA + H2O Xanthomonas campestris pv. campestris
-
 a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
 ?
an acyl-CoA + H2O Xanthomonas campestris pv. campestris ATCC 33913
-
 a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris pv. campestris Q8PDX2
-
-
Xanthomonas campestris pv. campestris ATCC 33913 Q8PDX2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-HisTrap column chromatography Xanthomonas campestris pv. campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 lauroyl-CoA + H2O
-
 Xanthomonas campestris pv. campestris (2R)-2-decanoyltetradecanoate + 2 CoA
-
 ?
2 lauroyl-CoA + H2O
-
 Xanthomonas campestris pv. campestris ATCC 33913 (2R)-2-decanoyltetradecanoate + 2 CoA
-
 ?
2 myristoyl-CoA + H2O
-
 Xanthomonas campestris pv. campestris (2R)-2-dodecanoylhexadecanoate + 2 CoA
-
 ?
2 myristoyl-CoA + H2O
-
 Xanthomonas campestris pv. campestris ATCC 33913 (2R)-2-dodecanoylhexadecanoate + 2 CoA
-
 ?
an acyl-CoA + H2O
-
 Xanthomonas campestris pv. campestris a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
 ?
an acyl-CoA + H2O
-
 Xanthomonas campestris pv. campestris ATCC 33913 a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 3866, calculated from amino acid sequence Xanthomonas campestris pv. campestris

Synonyms

Synonyms Comment Organism
oleA
-
 Xanthomonas campestris pv. campestris