Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.20 extracted from

  • Frias, J.; Richman, J.; Erickson, J.; Wackett, L.
    Purification and characterization of OleA from Xanthomonas campestris and demonstration of a non-decarboxylative claisen condensation reaction (2011), J. Biol. Chem., 286, 10930-10938 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli One Shot BL21(DE3) cells Xanthomonas campestris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 gel filtration Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
-
-
-
Xanthomonas campestris ATCC 33913
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanoyl-CoA + H2O 42% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
decanoyl-CoA + H2O 42% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
lauroyl-CoA + H2O 79% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
lauroyl-CoA + H2O 79% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
additional information no activity with acetyl-CoA Xanthomonas campestris ?
-
 ?
additional information no activity with acetyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
myristoyl-CoA + H2O 97% activity compared to palmitoyl-CoA Xanthomonas campestris 2-myristoylmyristic acid + myristic acid + 14-heptacosanone + CoA
-
 ?
myristoyl-CoA + H2O 97% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 2-myristoylmyristic acid + myristic acid + 14-heptacosanone + CoA
-
 ?
octanoyl-CoA + H2O 12% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
octanoyl-CoA + H2O 12% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
palmitoleoyl-CoA + H2O 57% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
palmitoyl-CoA + H2O 100% activity Xanthomonas campestris ?
-
 ?
stearoyl-CoA + H2O 29% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 36629, calculated from amino acid sequence Xanthomonas campestris
homodimer 2 * 40000, His-tagged enzyme, SDS-PAGE Xanthomonas campestris

Synonyms

Synonyms Comment Organism
oleA
-
 Xanthomonas campestris

pI Value

Organism Comment pI Value Maximum pI Value
Xanthomonas campestris calculated from amino acid sequence
-
 5.6