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Literature summary for 2.3.3.17 extracted from

  • de Kraker, J.W.; Gershenzon, J.
    From amino acid to glucosinolate biosynthesis: protein sequence changes in the evolution of methylthioalkylmalate synthase in Arabidopsis (2011), Plant Cell, 23, 38-53.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
 Arabidopsis thaliana
53200
-
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O Arabidopsis thaliana
-
 a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FG67 ecotype Columbia-0
-
Arabidopsis thaliana Q9FN52 ecotype Columbia-0
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylthio-2-oxobutyrate + acetyl-CoA + H2O
-
 Arabidopsis thaliana 2-(2'-methylthioethyl)malate + CoA
-
 ?
5-methylthio-2-oxopentanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana ?
-
 ?
6-methylthio-2-oxohexanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana 2-(4-methylthiobutyl)malate + CoA
-
 ?
an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, isoform MAM3, calculated from amino acid sequence Arabidopsis thaliana
monomer 1 * 53200, isoform MAM1, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
MAM1 isoform Arabidopsis thaliana
MAM3 isoform Arabidopsis thaliana