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Literature summary for 2.3.3.16 extracted from
- Citric acid cycle enzymes of the archaebacteria: citrate synthase and succinate thiokinase (1985), FEBS Lett., 179, 120-124.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Haloarcula vallismortis |  |
| 2-oxoglutarate | no inhibition | Halobacterium salinarum | |
| 2-oxoglutarate | - |
Haloferax volcanii | |
| 2-oxoglutarate | - |
Methanosarcina barkeri | |
| 2-oxoglutarate | - |
Natronobacterium gregoryi | |
| 2-oxoglutarate | - |
Natronococcus occultus | |
| 2-oxoglutarate | no inhibition | Natronomonas pharaonis | |
| 2-oxoglutarate | - |
Sulfolobus acidocaldarius | |
| 2-oxoglutarate | - |
Thermoplasma acidophilum | |
| ATP | - |
Haloarcula vallismortis | |
| ATP | - |
Halobacterium salinarum | |
| ATP | - |
Haloferax volcanii | |
| ATP | - |
Methanosarcina barkeri | |
| ATP | - |
Natronobacterium gregoryi | |
| ATP | - |
Natronococcus occultus | |
| ATP | - |
Natronomonas pharaonis | |
| ATP | - |
Sulfolobus acidocaldarius | |
| ATP | - |
Thermoplasma acidophilum | |
| NADH | - |
Halobacterium salinarum | |
| NADH | - |
Haloferax volcanii | |
| NADH | - |
Methanosarcina barkeri | |
| NADH | - |
Natronobacterium gregoryi | |
| NADH | - |
Natronomonas pharaonis | |
| NADH | - |
Sulfolobus acidocaldarius | |
| NADH | - |
Thermoplasma acidophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
oxaloacetate | - |
Thermoplasma acidophilum | |
| 0.006 | - |
acetyl-CoA | - |
Thermoplasma acidophilum | |
| 0.007 | - |
acetyl-CoA | - |
Methanosarcina barkeri | |
| 0.01 | - |
acetyl-CoA | - |
Sulfolobus acidocaldarius | |
| 0.018 | - |
oxaloacetate | - |
Methanosarcina barkeri | |
| 0.02 | - |
oxaloacetate | - |
Sulfolobus acidocaldarius | |
| 0.038 | - |
oxaloacetate | - |
Haloferax volcanii | |
| 0.058 | - |
oxaloacetate | - |
Halobacterium salinarum | |
| 0.063 | - |
oxaloacetate | - |
Natronobacterium gregoryi | |
| 0.075 | - |
acetyl-CoA | - |
Haloferax volcanii | |
| 0.11 | - |
acetyl-CoA | - |
Natronobacterium gregoryi | |
| 0.21 | - |
oxaloacetate | - |
Natronomonas pharaonis | |
| 0.211 | - |
acetyl-CoA | - |
Halobacterium salinarum | |
| 0.45 | - |
acetyl-CoA | - |
Natronomonas pharaonis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
gel filtration | Sulfolobus acidocaldarius |
| 85000 | - |
gel filtration | Thermoplasma acidophilum |
| 105000 | - |
gel filtration | Haloferax volcanii |
| 105000 | - |
gel filtration | Natronococcus occultus |
| 106000 | - |
gel filtration | Natronomonas pharaonis |
| 111000 | - |
gel filtration | Halobacterium salinarum |
| 112000 | - |
gel filtration | Haloarcula vallismortis |
| 112000 | - |
gel filtration | Natronobacterium gregoryi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloarcula vallismortis | - |
- |
- |
| Halobacterium salinarum | - |
- |
- |
| Haloferax volcanii | - |
- |
- |
| Methanosarcina barkeri | - |
- |
- |
| Natronobacterium gregoryi | - |
- |
- |
| Natronococcus occultus | - |
- |
- |
| Natronomonas pharaonis | - |
- |
- |
| Sulfolobus acidocaldarius | - |
- |
- |
| Thermoplasma acidophilum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.002 | - |
- |
Methanosarcina barkeri |
| 0.014 | - |
- |
Natronobacterium gregoryi |
| 0.026 | - |
- |
Natronomonas pharaonis |
| 0.051 | - |
- |
Halobacterium salinarum |
| 0.061 | - |
- |
Haloferax volcanii |
| 0.172 | - |
- |
Thermoplasma acidophilum |
| 0.28 | - |
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Methanosarcina barkeri | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Halobacterium salinarum | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Haloarcula vallismortis | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Haloferax volcanii | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Natronomonas pharaonis | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Natronobacterium gregoryi | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Natronococcus occultus | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Sulfolobus acidocaldarius | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Thermoplasma acidophilum | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Sulfolobus acidocaldarius DSM 639 | citrate + CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Natronomonas pharaonis |
| 30 | - |
enzyme assay at | Methanosarcina barkeri |
| 30 | - |
enzyme assay at | Halobacterium salinarum |
| 30 | - |
enzyme assay at | Haloferax volcanii |
| 30 | - |
enzyme assay at | Natronobacterium gregoryi |
| 55 | - |
enzyme assay at | Sulfolobus acidocaldarius |
| 55 | - |
enzyme assay at | Thermoplasma acidophilum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
NADH | below | Methanosarcina barkeri | |
| 0.3 | - |
ATP | below | Methanosarcina barkeri | |
| 0.3 | - |
2-oxoglutarate | below | Methanosarcina barkeri | |
| 0.9 | - |
ATP | - |
Sulfolobus acidocaldarius | |
| 1.9 | - |
ATP | - |
Haloferax volcanii | |
| 2.2 | - |
ATP | - |
Thermoplasma acidophilum | |
| 2.2 | - |
2-oxoglutarate | - |
Sulfolobus acidocaldarius | |
| 3.2 | - |
NADH | - |
Natronobacterium gregoryi | |
| 4.2 | - |
ATP | - |
Natronobacterium gregoryi | |
| 4.6 | - |
NADH | - |
Sulfolobus acidocaldarius | |
| 5.2 | - |
NADH | - |
Natronomonas pharaonis | |
| 5.4 | - |
NADH | - |
Thermoplasma acidophilum | |
| 5.4 | - |
2-oxoglutarate | - |
Thermoplasma acidophilum | |
| 5.6 | - |
ATP | - |
Halobacterium salinarum | |
| 10 | - |
NADH | above | Halobacterium salinarum | |
| 10 | - |
NADH | above | Haloferax volcanii | |
| 20 | - |
2-oxoglutarate | above | Haloferax volcanii | |
| 20 | - |
2-oxoglutarate | above | Natronobacterium gregoryi | |
| 30 | - |
ATP | above | Natronomonas pharaonis | |
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