Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | 0.1 M, 92% inhibition of wild-type, 25% inhibition of K283 acetylated variant, 88% inhibition of K295 acetylated variant, 7% inhibition of K168 acetylated variant | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
oxaloacetate | wild-type, pH 7.8, 25°C | Escherichia coli | |
0.023 | - |
oxaloacetate | K283 acetylated variant, pH 7.8, 25°C | Escherichia coli | |
0.025 | - |
oxaloacetate | K168 acetylated variant, pH 7.8, 25°C | Escherichia coli | |
0.032 | - |
acetyl-CoA | K283 acetylated variant, pH 7.8, 25°C | Escherichia coli | |
0.032 | - |
oxaloacetate | K295 acetylated variant, pH 7.8, 25°C | Escherichia coli | |
0.098 | - |
acetyl-CoA | wild-type, pH 7.8, 25°C | Escherichia coli | |
0.145 | - |
acetyl-CoA | K168 acetylated variant, pH 7.8, 25°C | Escherichia coli | |
0.478 | - |
acetyl-CoA | K295 acetylated variant, pH 7.8, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Escherichia coli | citrate + CoA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56 | - |
oxaloacetate | K295 acetylated variant, pH 7.8, 25°C | Escherichia coli | |
89 | - |
oxaloacetate | K168 acetylated variant, pH 7.8, 25°C | Escherichia coli | |
92 | - |
oxaloacetate | wild-type, pH 7.8, 25°C | Escherichia coli | |
114 | - |
oxaloacetate | K283 acetylated variant, pH 7.8, 25°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | recombinant wild-type CS has no detectable acetylation. Acetylation of lysine residues does not result in significantly different activities with that of the wild-type, except for residues K283 and K295. Acetylation at K283 increases the activity by nearly twofold, while acetylation at K295 decreased the activity by about 10fold. CS can be acetylated by acetyl-phosphate chemically, and be deacetylated by the CobB deacetylase | Escherichia coli |