Literature summary for 2.3.3.16 extracted from

Li, D.; Pang, L.; Yuan, P.; Zheng, P.; Huai, B.; Yao, M.; Kang, Z.; Liu, J.
A novel citrate synthase isoform contributes infection and stress resistance of the stripe rust fungus (2018), Environ. Microbiol., 20, 4037-4050 .

Search for more literature for 2.3.3.16

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Puccinia striiformis f. sp. tritici

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	87% decrease in activity	Puccinia striiformis f. sp. tritici

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Puccinia striiformis f. sp. tritici	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	0.5 mM, 20% increase in activity	Puccinia striiformis f. sp. tritici

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
494770	-	gel filtration	Puccinia striiformis f. sp. tritici

Organism

Organism	UniProt	Comment	Textmining
Puccinia striiformis f. sp. tritici	-	-	-

Subunits

Subunits	Comment	Organism
octamer	8 * 55001, calculated from sequence, 8 * 70000, SDS-PAGE of recombinant protein	Puccinia striiformis f. sp. tritici

Synonyms

Synonyms	Comment	Organism
CS1	-	Puccinia striiformis f. sp. tritici

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	-	Puccinia striiformis f. sp. tritici

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Puccinia striiformis f. sp. tritici

pI Value

Organism	Comment	pI Value Maximum	pI Value
Puccinia striiformis f. sp. tritici	calculated from sequence	-	9.1

Expression

Organism	Comment	Expression
Puccinia striiformis f. sp. tritici	CS1 is expressed differentially in nongerminated urediniospores and Pst-infected wheat leaves. The enzyme activity significantly increases upon germination compared with nongerminated urediniospores	additional information

General Information

General Information	Comment	Organism
physiological function	overexpression of CS1 in Escherichia coli enhances its growth under salt stress. Silencing of CS1 reduces fungal biomass at the middle stage of Puccinia striiformis infection, restricts fungal growth and development	Puccinia striiformis f. sp. tritici

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Release 2023.1 (January 2023)