Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
wild type enzyme bound to calcium by the hanging drop vapor diffusion method, using 0.2 M calcium acetate, 0.1 M imidazole (pH 9.0), and 4-6% (w/v) PEG 8000, and an active site variant E151Q in complex with oxaloacetate by the sitting drop vapor diffusion method, using 5% (v/v) tascimate (pH 7.0), 0.1 M HEPES (pH 7.0), 10% (w/v) PEG monomethyl ether 5000 and about 40 mM guanidine hydrochloride | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D177A | the mutant shows reduced activity compared to the wild type enzyme | Staphylococcus aureus |
E151Q | the mutant shows reduced activity compared to the wild type enzyme | Staphylococcus aureus |
E46Q | the mutant shows reduced activity compared to the wild type enzyme | Staphylococcus aureus |
H47A | the mutant shows reduced activity compared to the wild type enzyme | Staphylococcus aureus |
H96A | the mutant shows reduced activity compared to the wild type enzyme | Staphylococcus aureus |
R72A | the mutant shows reduced activity compared to the wild type enzyme | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Staphylococcus aureus | |
Mg2+ | - |
Staphylococcus aureus | |
additional information | not inhibited by EDTA | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
6 * 28000, SDS-PAGE | Staphylococcus aureus |
171000 | - |
multi-angle light scattering | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | Staphylococcus aureus | - |
citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A0A0H3K9Z0 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Source 15Q column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Staphylococcus aureus | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 28000, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
SbnG | - |
Staphylococcus aureus |