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Literature summary for 2.3.3.16 extracted from

  • Kobylarz, M.J.; Grigg, J.C.; Sheldon, J.R.; Heinrichs, D.E.; Murphy, M.E.
    SbnG, a citrate synthase in Staphylococcus aureus: a new fold on an old enzyme (2014), J. Biol. Chem., 289, 33797-33807.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdaDE3) cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type enzyme bound to calcium by the hanging drop vapor diffusion method, using 0.2 M calcium acetate, 0.1 M imidazole (pH 9.0), and 4-6% (w/v) PEG 8000, and an active site variant E151Q in complex with oxaloacetate by the sitting drop vapor diffusion method, using 5% (v/v) tascimate (pH 7.0), 0.1 M HEPES (pH 7.0), 10% (w/v) PEG monomethyl ether 5000 and about 40 mM guanidine hydrochloride Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
D177A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
E151Q the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
E46Q the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
H47A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
H96A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
R72A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Staphylococcus aureus
Mg2+
-
Staphylococcus aureus
additional information not inhibited by EDTA Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
6 * 28000, SDS-PAGE Staphylococcus aureus
171000
-
multi-angle light scattering Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate Staphylococcus aureus
-
citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A0H3K9Z0
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Source 15Q column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Staphylococcus aureus citrate + CoA
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 28000, SDS-PAGE Staphylococcus aureus

Synonyms

Synonyms Comment Organism
SbnG
-
Staphylococcus aureus