Protein Variants | Comment | Organism |
---|---|---|
G181E | the mutant shows reduced activity compared to the wild type enzyme and is not inhibited by NADH | Escherichia coli |
R306L | inactive | Escherichia coli |
T204R | the mutant shows reduced activity compared to the wild type enzyme and is not inhibited by NADH | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | about 95% inhibition at 0.1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0105 | - |
oxaloacetate | mutant enzyme G181E, pH and temperature not specified in the publication | Escherichia coli | |
0.011 | - |
oxaloacetate | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
0.69 | - |
acetyl-CoA | mutant enzyme G181E, pH and temperature not specified in the publication | Escherichia coli | |
0.75 | - |
acetyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
1.01 | - |
acetyl-CoA | mutant enzyme T204R, pH and temperature not specified in the publication | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47885 | - |
6 * 47885, calculated from amino acid sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | Escherichia coli | - |
citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABH7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Escherichia coli | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 47885, calculated from amino acid sequence | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
acetyl-CoA | mutant enzyme T204R, pH and temperature not specified in the publication | Escherichia coli | |
18 | - |
acetyl-CoA | mutant enzyme G181E, pH and temperature not specified in the publication | Escherichia coli | |
44 | - |
acetyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |