Cloned (Comment) | Organism |
---|---|
cloning and expression of citA in Aspergillus niger, 11fold overexpression does not increase the citrate activity level in vivo | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
oxaloacetate | pH 7.5 | Aspergillus niger | |
0.014 | - |
acetyl-CoA | pH 7.5 | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | N-terminal mitochondrial import signal sequence | Aspergillus niger | 5739 | - |
additional information | N-terminal mitochondrial signal and C-terminal peroxisomal target sequence, unclear if both are functional | Aspergillus niger | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
C-terminal amino acid sequence alignment | Aspergillus niger |
additional information | - |
N-terminal protein sequence alignment | Aspergillus niger |
48000 | - |
2 * 48000, mitochondrial enzyme, SDS-PAGE | Aspergillus niger |
80000 | - |
gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | Aspergillus niger | contributes little to flux control in the pathway | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 | contributes little to flux control in the pathway | citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | Q9P8D6 | gene citA | - |
Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 | Q9P8D6 | gene citA | - |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79 | - |
purified enzyme | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Aspergillus niger | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | contributes little to flux control in the pathway | Aspergillus niger | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | contributes little to flux control in the pathway | Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, mitochondrial enzyme, SDS-PAGE | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Aspergillus niger |