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Literature summary for 2.3.3.16 extracted from

  • Ruijter, G.J.G.; Panneman, H.; Xu, D.B.; Visser, J.
    Properties of Aspergillus niger citrate synthase and effects of citA overexpression on citric acid production (2000), FEMS Microbiol. Lett., 184, 35-40.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of citA in Aspergillus niger, 11fold overexpression does not increase the citrate activity level in vivo Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
oxaloacetate pH 7.5 Aspergillus niger
0.014
-
acetyl-CoA pH 7.5 Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion N-terminal mitochondrial import signal sequence Aspergillus niger 5739
-
additional information N-terminal mitochondrial signal and C-terminal peroxisomal target sequence, unclear if both are functional Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
C-terminal amino acid sequence alignment Aspergillus niger
additional information
-
N-terminal protein sequence alignment Aspergillus niger
48000
-
2 * 48000, mitochondrial enzyme, SDS-PAGE Aspergillus niger
80000
-
gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O Aspergillus niger contributes little to flux control in the pathway citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 contributes little to flux control in the pathway citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger Q9P8D6 gene citA
-
Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 Q9P8D6 gene citA
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
79
-
purified enzyme Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Aspergillus niger citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O contributes little to flux control in the pathway Aspergillus niger citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O contributes little to flux control in the pathway Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49 citrate + CoA
-
?

Subunits

Subunits Comment Organism
dimer 2 * 48000, mitochondrial enzyme, SDS-PAGE Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Aspergillus niger