Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | no inhibition | Methylorubrum extorquens | |
ADP | no inhibition | Methylorubrum extorquens | |
ATP | - |
Methylorubrum extorquens | |
NADH | no effect | Methylorubrum extorquens | |
NADPH | no inhibition | Methylorubrum extorquens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
oxaloacetate | - |
Methylorubrum extorquens | |
0.084 | - |
acetyl-CoA | - |
Methylorubrum extorquens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | up to 80 mM | Methylorubrum extorquens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
4 * 66000, SDS-PAGE | Methylorubrum extorquens |
260000 | - |
gel filtration | Methylorubrum extorquens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | Methylorubrum extorquens | involved in poly 3-hydroxybutyrate synthesis | citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylorubrum extorquens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24 | - |
purified enzyme | Methylorubrum extorquens |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl buffer, pH8.0, 50 mM glycerol, at least 1 week | Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Methylorubrum extorquens | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | involved in poly 3-hydroxybutyrate synthesis | Methylorubrum extorquens | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 66000, SDS-PAGE | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
CS | - |
Methylorubrum extorquens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme assay at | Methylorubrum extorquens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | 9 | - |
Methylorubrum extorquens |