Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant in Escherichia coli | Thermoplasma acidophilum |
Protein Variants | Comment | Organism |
---|---|---|
S43C | site-directed mutagenesis, 5.7fold reduced activity, unaltered Km values for the substrates and unaltered thermostability | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
oxaloacetate | recombinant wild-type and mutant S34C | Thermoplasma acidophilum | |
0.011 | - |
acetyl-CoA | recombinant wild-type and mutant S43C | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant wild-type, the latter from E. coli and recombinant S43C mutant enzyme from E. coli | Thermoplasma acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9 | - |
S43C mutant | Thermoplasma acidophilum |
51.4 | - |
wild-type | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Thermoplasma acidophilum | citrate + CoA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
effect of C43 mutation | Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant wild-type and mutant | Thermoplasma acidophilum |