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Literature summary for 2.3.3.16 extracted from

  • Kocabryik, S.; Erduran, I.; Russell, R.J.M.; Danson, M.J.; Hough, D.W.
    The effect of C43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum (1996), Biochem. Biophys. Res. Commun., 224, 224-228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant in Escherichia coli Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
S43C site-directed mutagenesis, 5.7fold reduced activity, unaltered Km values for the substrates and unaltered thermostability Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
oxaloacetate recombinant wild-type and mutant S34C Thermoplasma acidophilum
0.011
-
acetyl-CoA recombinant wild-type and mutant S43C Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant wild-type, the latter from E. coli and recombinant S43C mutant enzyme from E. coli Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9
-
S43C mutant Thermoplasma acidophilum
51.4
-
wild-type Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Thermoplasma acidophilum citrate + CoA
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
effect of C43 mutation Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant wild-type and mutant Thermoplasma acidophilum