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Literature summary for 2.3.3.16 extracted from
- Characterization of citrate synthase purified from Drosophila melanogaster (1997), Mol. Cells, 7, 599-604.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | competitive against oxaloacetate | Drosophila melanogaster |  |
| ATP | competitive against acetyl-CoA | Drosophila melanogaster | |
| additional information | tricarboxylic acid cycle intermediates | Drosophila melanogaster | |
| propionyl-CoA | competitive against acetyl-CoA | Drosophila melanogaster | |
| succinyl-CoA | mixed-type inhibition | Drosophila melanogaster | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0031 | - |
oxaloacetate | - |
Drosophila melanogaster | |
| 0.0067 | - |
acetyl-CoA | - |
Drosophila melanogaster | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Drosophila melanogaster |
| 48700 | - |
2 * 48700, SDS-PAGE | Drosophila melanogaster |
| 81000 | - |
gel filtration | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| antibody studies | Drosophila melanogaster |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate = citrate + CoA | mechanism | Drosophila melanogaster |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 112.5 | - |
purified enzyme | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Drosophila melanogaster | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48700, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Drosophila melanogaster |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
propionyl-CoA | versus acetyl-CoA, competitive | Drosophila melanogaster | |
| 0.0225 | - |
propionyl-CoA | versus oxaloacetate, noncompetitive | Drosophila melanogaster | |
| 0.125 | - |
ATP | versus acetyl-CoA | Drosophila melanogaster | |
| 0.38 | - |
2-oxoglutarate | versus oxaloacetate, competitive | Drosophila melanogaster | |
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