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Literature summary for 2.3.3.16 extracted from
- Citrate synthase from the hyperthermophilic archaeon, Pyrococcus furiosus (1995), Protein Eng., 8, 583-592.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli JM105, DNA and amino acid sequence analysis | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
acetyl-CoA | recombinant enzyme | Pyrococcus furiosus |  |
| 0.005 | - |
oxaloacetate | recombinant enzyme | Pyrococcus furiosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 5fold increase at 100-200 mM | Pyrococcus furiosus | |
| NaCl | 5fold increase at 100-200 mM | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence alignment | Pyrococcus furiosus |
| additional information | - |
secondary and tertiary structure | Pyrococcus furiosus |
| 42600 | - |
2 * 42600, SDS-PAGE | Pyrococcus furiosus |
| 89700 | - |
gel filtration | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from E. coli | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified recombinant enzyme from E. coli | Pyrococcus furiosus |
| 112.5 | - |
recombinant enzyme | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Pyrococcus furiosus | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42600, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
enzyme assay at | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
half-life: 20 min | Pyrococcus furiosus |
| 104 | - |
half-life: 1 min | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
enzyme assay at | Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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