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Literature summary for 2.3.3.16 extracted from

  • Otto, R.
    Regulatory and molecular properties of citrate synthases from methylotrophs (1986), FEMS Microbiol. Lett., 34, 191-194.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Bacillus subtilis
NADH not, only gram-negative facultative methylotrophs Arthrobacter sp.
NADH not, only gram-negative facultative methylotrophs Hyphomicrobium sp.
NADH not, only gram-negative facultative methylotrophs Methylophilus methylotrophus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Arthrobacter sp.
additional information
-
additional information influence of NADH and AMP on Km for acetyl-CoA Hyphomicrobium sp.
0.143 0.161 acetyl-CoA strains X and Hanham Hyphomicrobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
small and large enzyme Bacillus subtilis
additional information
-
small and large enzyme Arthrobacter sp.
additional information
-
small and large enzyme Methylophilus methylotrophus
additional information
-
small and large enzyme Hyphomicrobium sp.
150000
-
above: gram-negative methylotrophs, below: gram-positive methylotrophs Arthrobacter sp.
150000
-
above: gram-negative methylotrophs, below: gram-positive methylotrophs Methylophilus methylotrophus
150000
-
above: gram-negative methylotrophs, below: gram-positive methylotrophs Hyphomicrobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O Bacillus subtilis
-
citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O Arthrobacter sp. only anabolic pathway citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O Methylophilus methylotrophus only anabolic pathway citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O Hyphomicrobium sp. only anabolic pathway citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O Bacillus subtilis S2A1
-
citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O Arthrobacter sp. 2B2 only anabolic pathway citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. 2B2
-
-
-
Bacillus subtilis
-
-
-
Bacillus subtilis S2A1
-
-
-
Hyphomicrobium sp.
-
strains X and Hanham
-
Methylophilus methylotrophus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Bacillus subtilis citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Arthrobacter sp. citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Methylophilus methylotrophus citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Hyphomicrobium sp. citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O only anabolic pathway Arthrobacter sp. citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O only anabolic pathway Methylophilus methylotrophus citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O only anabolic pathway Hyphomicrobium sp. citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Bacillus subtilis S2A1 citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Arthrobacter sp. 2B2 citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O only anabolic pathway Arthrobacter sp. 2B2 citrate + CoA
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4 12 NADH strains X and Hanham Hyphomicrobium sp.