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Literature summary for 2.3.3.16 extracted from
- Properties of citrate synthase from Pisum sativum mitochondria (1979), Phytochemistry, 18, 1057.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | no inhibition | Pisum sativum |  |
| ADP | 25% inhibition at 5 mM | Pisum sativum | |
| AMP | 8% inhibition at 5 mM | Pisum sativum | |
| ATP | 50% inhibition at 5 mM, competitive against acetyl-CoA; feed back inhibition | Pisum sativum | |
| HgCl2 | weak inhibition | Pisum sativum | |
| NADH | no effect | Pisum sativum | |
| p-hydroxymercuribenzoate | 60% inhibition at 0.1 mM, protection by oxaloacetate | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
oxaloacetate | - |
Pisum sativum | |
| 0.031 | - |
acetyl-CoA | - |
Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Pisum sativum | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | Pisum sativum | entry step to tricarboxylic acid cycle | citrate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epicotyl | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Pisum sativum | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | entry step to tricarboxylic acid cycle | Pisum sativum | citrate + CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Pisum sativum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
ATP | - |
Pisum sativum | |
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