Literature summary for 2.3.3.16 extracted from

Porter, J.S.; Wright, B.E.
Partial purification and characterization of citrate synthase from Dictyostelium discoideum (1977), Arch. Biochem. Biophys., 181, 155-163.

Search for more literature for 2.3.3.16

Activating Compound

Activating Compound	Comment	Organism	Structure
ammonium sulfate	activates at 0.5 mM	Dictyostelium discoideum

General Stability

General Stability	Organism
destabilization by all salts, exception is ammonium sulfate, which stabilizes at low concentration	Dictyostelium discoideum
dithiothreitol stabilizes for about 5 days	Dictyostelium discoideum

Inhibitors

Inhibitors	Comment	Organism	Structure
2-oxoglutarate	no inhibition	Dictyostelium discoideum
acetyl-CoA	no inhibition	Dictyostelium discoideum
ATP	no inhibition	Dictyostelium discoideum
CoA	competitive with acetyl-CoA, noncompetitive with oxaloacetate	Dictyostelium discoideum
NADH	no effect	Dictyostelium discoideum
oxaloacetate	no inhibition	Dictyostelium discoideum
p-chloromercuribenzoate	can be restored by dithiothreitol treatment	Dictyostelium discoideum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.007	-	oxaloacetate	-	Dictyostelium discoideum
0.01	-	acetyl-CoA	-	Dictyostelium discoideum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
(NH4)2SO4	activates at at least 0.5 mM	Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	gel filtration	Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + oxaloacetate + H2O	Dictyostelium discoideum	key enzyme of tricarboxylic acid cycle	citrate + CoA	-	r
acetyl-CoA + oxaloacetate + H2O	Dictyostelium discoideum NC-4 / ATCC 24697	key enzyme of tricarboxylic acid cycle	citrate + CoA	-	r

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	-	-	-
Dictyostelium discoideum NC-4 / ATCC 24697	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Dictyostelium discoideum

Reaction

Reaction	Comment	Organism	Reaction ID
acetyl-CoA + H2O + oxaloacetate = citrate + CoA	ordered sequential mechanism	Dictyostelium discoideum	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
471.7	-	partially purified enzyme	Dictyostelium discoideum

Storage Stability

Storage Stability	Organism
0°C, below, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol and 5 mg/ml bovine serum albumin, several months	Dictyostelium discoideum
4°C, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol, 1 day	Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + oxaloacetate + H2O	-	Dictyostelium discoideum	citrate + CoA	-	r
acetyl-CoA + oxaloacetate + H2O	key enzyme of tricarboxylic acid cycle	Dictyostelium discoideum	citrate + CoA	-	r
acetyl-CoA + oxaloacetate + H2O	-	Dictyostelium discoideum NC-4 / ATCC 24697	citrate + CoA	-	r
acetyl-CoA + oxaloacetate + H2O	key enzyme of tricarboxylic acid cycle	Dictyostelium discoideum NC-4 / ATCC 24697	citrate + CoA	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
23	-	enzyme assay at	Dictyostelium discoideum

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.2	9	-	Dictyostelium discoideum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.11	-	CoA	-	Dictyostelium discoideum

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Release 2023.1 (January 2023)