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Literature summary for 2.3.3.16 extracted from
- Some properties of the citrate synthase from the extreme halophile, Halobacterium cutirubrum (1975), Biochem. J., 147, 267-274.
General Stability
| General Stability | Organism |
|---|---|
| dilute solutions instable, best stabilized by high concentrations of NaCl or KCl | Halobacterium salinarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | low salt concentrations inactivate reversibly | Halobacterium salinarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km increases 5fold with salt concentration from 1 to 3 M, overview | Halobacterium salinarum | |
| 0.018 | - |
oxaloacetate | minimum at 1 M KCl | Halobacterium salinarum |  |
| 0.26 | - |
acetyl-CoA | minimum at 1 M KCl | Halobacterium salinarum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | as CaCl2, inhibition at concentrations higher than 50 mM | Halobacterium salinarum | |
| K+ | activation, Cl- most efficient anion | Halobacterium salinarum | |
| KCl | maximal activation at 3 M | Halobacterium salinarum | |
| Mg2+ | as MgCl2, inhibition at concentrations higher than 50 mM | Halobacterium salinarum | |
| NaCl | - |
Halobacterium salinarum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Halobacterium salinarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Halobacterium salinarum |
| 27.45 | - |
- |
Halobacterium salinarum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, Tris-HCl buffer, pH 7.6, high protein concentration, below 20% loss of activity in 2 months | Halobacterium salinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Halobacterium salinarum | citrate + CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme assay at | Halobacterium salinarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
cold-lability in presence of 3 M KCl, but not with NaCl up to 5 M | Halobacterium salinarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Halobacterium salinarum |
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Release 2023.1 (January 2023)
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