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Literature summary for 2.3.3.14 extracted from

  • Jaklitsch, W.M.; Kubicek, C.P.
    Homocitrate synthase from Penicillium chrysogenum (1990), Biochem. J., 269, 247-253.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.14

General Stability

General Stability Organism
enzyme is very unstable in crude and protamine sulphate-treated desalted extracts Penicillium chrysogenum
freezing and thawing results in 50% loss of activity Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
AMP 1 mM, 13-15% inhibition Penicillium chrysogenum
CoA
-
 Penicillium chrysogenum
EDTA 5 mM, 30% inhibition Penicillium chrysogenum
L-Lys 50% inhibition by 0.053 mM, at 6 mM 2-oxoglutarate Penicillium chrysogenum
Mg2+ 10 mM MgSO4, 30% inhibition Penicillium chrysogenum
Mn2+ 0.025 mM, 35% inhibition Penicillium chrysogenum
Zn2+ 0.025 mM, 60% inhibition Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 2-oxoglutarate
-
 Penicillium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol main activity located in Penicillium chrysogenum 5829
-
mitochondrion 25% of total activity Penicillium chrysogenum 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
 gel filtration Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Penicillium chrysogenum

Storage Stability

Storage Stability Organism
4-6°C, enzyme in cell-free extract in presence of 20% glycerol, 65% loss of activity after 48 h Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate
-
 Penicillium chrysogenum 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Penicillium chrysogenum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
 L-Lys
-
 Penicillium chrysogenum