Cloned (Comment) | Organism |
---|---|
the coding sequences of alpha-IPMS-2CR and alpha-IPMS-14CR are cloned into the vector pET15b for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
Y410F | mutant, insensitive to feedback inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
acetyl-CoA | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
0.035 | - |
alpha-ketoisovaleric acid | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
0.261 | - |
alpha-ketoisovaleric acid | alpha-IPMS-2CR | Mycobacterium tuberculosis | |
0.568 | - |
acetyl-CoA | alpha-IPMS-2CR | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
alpha-IPMS-2CR, determined by SDS-PAGE | Mycobacterium tuberculosis |
99000 | - |
alpha-IPMS-14CR, determined by SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis | - |
(2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis H37Rv | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQB3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WQB3 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA resin | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis H37Rv | (2S)-2-isopropylmalate + CoA | - |
? | |
alpha-ketoisovaleric acid + H2O + acetyl-CoA | - |
Mycobacterium tuberculosis | ? + CoA | - |
? | |
alpha-ketoisovaleric acid + H2O + acetyl-CoA | - |
Mycobacterium tuberculosis H37Rv | ? + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 74000, alpha-IPMS-2CR, 2 * 99000, alpha-IPMS-14CR, determined by gel filtration | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
alpha-IPMS | - |
Mycobacterium tuberculosis |
alpha-IPMS-14CR | - |
Mycobacterium tuberculosis |
alpha-IPMS-14CR | the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-14CR comprises 14 copies | Mycobacterium tuberculosis |
alpha-IPMS-2CR | the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-2CR comprises two copies | Mycobacterium tuberculosis |
Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 42 | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
alpha-ketoisovaleric acid | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
0.61 | - |
acetyl-CoA | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
1.17 | - |
alpha-ketoisovaleric acid | alpha-IPMS-2CR | Mycobacterium tuberculosis | |
2.22 | - |
acetyl-CoA | alpha-IPMS-2CR | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity assay | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mycobacterium tuberculosis |