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Literature summary for 2.3.3.13 extracted from
- Leucine synthesis in spinach chloroplasts: partial characterization of 2-isopropylmalate synthase (1993), Biol. Chem. Hoppe-Seyler, 374, 1105-1108.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| leucine | - |
Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
acetyl-CoA | - |
Spinacia oleracea | |
| 0.075 | - |
2-oxo-3-methylbutanoate | - |
Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates, maximal activity at 2 mM | Spinacia oleracea | |
| Na+ | stimulates, maximal activity at 1 mM | Spinacia oleracea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Spinacia oleracea | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | pH 6.0: about 50% of maximal activity, pH 9.5: about 80% of maximal activity | Spinacia oleracea |
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Release 2023.1 (January 2023)
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